Application | Comment | Organism |
---|---|---|
synthesis | sucrose is converted to alpha,alpha-trehalose in about 60% yield using a coupled enzyme system composed of sucrose phosphorylase from Leuconostoc mesenteroides, glucose isomerase from Streptomyces murinus and the appropriately stabilized PoTPase | Pleurotus ostreatus |
General Stability | Organism |
---|---|
unstable protein, half-life of no more than approximately 1.3 h in 20mM MES buffer, 25°C, pH 7.0. The enzyme can be stabilized on addition of alpha,alpha-trehalose (300 mM) which yields a half-life of 11.5 h. In the presence of 20% (w/v) glycerol, and 9 and 26% (w/v) PEG-4000, half-life is 6.5 h, 16 h and 70 h, respectively. Large improvements in stability by covalent protein modification with activated derivatives of PEG-5000 | Pleurotus ostreatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
vanadate | - |
Pleurotus ostreatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.5 | - |
phosphate | - |
Pleurotus ostreatus | |
3.5 | - |
phosphate | 30°C, pH 6.8 | Pleurotus ostreatus | |
4.1 | - |
alpha-D-glucose 1-phosphate | - |
Pleurotus ostreatus | |
4.1 | - |
alpha-D-glucose 1-phosphate | 30°C, pH 6.8 | Pleurotus ostreatus | |
40 | - |
alpha-D-glucose | - |
Pleurotus ostreatus | |
40 | - |
alpha-D-glucose | 30°C, pH 6.8 | Pleurotus ostreatus | |
79 | - |
alpha,alpha-trehalose | - |
Pleurotus ostreatus | |
79 | - |
alpha,alpha-trehalose | 30°C, pH 6.8 | Pleurotus ostreatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
SDS PAGE | Pleurotus ostreatus |
58300 | - |
gel filtration | Pleurotus ostreatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pleurotus ostreatus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pleurotus ostreatus |
trehalose phosphorylase from the basidiomycete Pleurotus ostreatus (PoTPase) is isolated from fungal fruit bodies through 500-fold purification with a yield of 44% | Pleurotus ostreatus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit body | - |
Pleurotus ostreatus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pleurotus ostreatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-dehydro-D-glucose + alpha-D-glucose 1-phosphate | - |
Pleurotus ostreatus | ? + phosphate | - |
? | |
2-deoxy-D-glucose + alpha-D-glucose 1-phosphate | 8% of the activity with alpha-D-glucose | Pleurotus ostreatus | ? | - |
r | |
2-deoxy-D-glucose + alpha-D-glucose 1-phosphate | - |
Pleurotus ostreatus | ? + phosphate | - |
? | |
2-fluoro-D-glucose + alpha-D-glucose 1-phosphate | 15% of the activity with alpha-D-glucose | Pleurotus ostreatus | ? | - |
r | |
2-fluoro-D-glucose + alpha-D-glucose 1-phosphate | - |
Pleurotus ostreatus | ? + phosphate | - |
? | |
2-keto-D-glucose + alpha-D-glucose 1-phosphate | 50% of the activity with alpha-D-glucose | Pleurotus ostreatus | ? | - |
r | |
alpha,alpha-trehalose + arsenate | 30% of the activity with phosphate | Pleurotus ostreatus | alpha-D-glucose 1-arsenate + alpha-D-glucose | - |
? | |
alpha,alpha-trehalose + arsenate | - |
Pleurotus ostreatus | alpha-D-glucose + alpha-D-glucose 1-arsenate | - |
r | |
alpha,alpha-trehalose + phosphate | - |
Pleurotus ostreatus | alpha-D-glucose + alpha-D-glucose 1-phosphate | - |
r | |
alpha-D-glucose + alpha-D-glucose 1-phosphate | beta-D-glucose 1-phosphate is not turned over by the enzyme | Pleurotus ostreatus | alpha,alpha-trehalose + phosphate | - |
r | |
D-mannose + alpha-D-glucose 1-phosphate | 3% of the activity with alpha-D-glucose | Pleurotus ostreatus | ? | - |
r | |
D-mannose + alpha-D-glucose 1-phosphate | - |
Pleurotus ostreatus | ? + phosphate | - |
? | |
additional information | no measurable activity is found with D-arabinose, D-fructose, D-fucose, D-galactose, D-lyxose, D-mannoheptaose, D-ribose and D-xylose | Pleurotus ostreatus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE | Pleurotus ostreatus |
monomer | gel filtration | Pleurotus ostreatus |
Synonyms | Comment | Organism |
---|---|---|
PoTPase | - |
Pleurotus ostreatus |
trehalose phosphorylase | - |
Pleurotus ostreatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Pleurotus ostreatus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
PoTPase has a half life of approximately 1 h at 30°C | Pleurotus ostreatus |
30 | - |
half-life: approximately 1 h in absence of stabilizing compound, 11.5 h in presence of 300 mM alpha,alpha-trehalose, 6.5 h in presence of 20% w/v glycerol, 70 h in presence of 26% w/v polyethylenglycol. Covalent modification of PoTPase with activated derivatives of PEG 5000 increases the stability by up to 600fold | Pleurotus ostreatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.27 | - |
2-deoxy-D-glucose | - |
Pleurotus ostreatus | |
0.42 | - |
D-mannose | - |
Pleurotus ostreatus | |
0.89 | - |
2-fluoro-D-glucose | - |
Pleurotus ostreatus | |
1.15 | - |
arsenate | - |
Pleurotus ostreatus | |
4.5 | - |
2-dehydro-D-glucose | - |
Pleurotus ostreatus | |
16 | - |
alpha-D-glucose 1-phosphate | - |
Pleurotus ostreatus | |
16 | - |
alpha-D-glucose 1-phosphate | 30°C, pH 6.8 | Pleurotus ostreatus | |
16.8 | - |
alpha-D-glucose | - |
Pleurotus ostreatus | |
16.8 | - |
alpha-D-glucose | 30°C, pH 6.8 | Pleurotus ostreatus | |
17.6 | - |
phosphate | - |
Pleurotus ostreatus | |
17.6 | - |
phosphate | 30°C, pH 6.8 | Pleurotus ostreatus | |
18.3 | - |
alpha,alpha-trehalose | - |
Pleurotus ostreatus | |
18.3 | - |
alpha,alpha-trehalose | 30°C, pH 6.8 | Pleurotus ostreatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | 6.4 | synthesis of alpha,alpha-trehalose | Pleurotus ostreatus |
6.2 | - |
alpha, alpha-trehalose synthesis | Pleurotus ostreatus |
6.8 | - |
assay at | Pleurotus ostreatus |
6.8 | - |
alpha, alpha-trehalose phosphorolysis | Pleurotus ostreatus |
6.8 | 7.2 | phosphorolysis | Pleurotus ostreatus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 7.8 | pH 5.5: about 75% of maximal activity, pH 7.8: about 80% of maxuimal activity, phosphorolysis | Pleurotus ostreatus |
5.5 | 6.8 | pH 5.5: about 80% of maximal activity, pH 75% of maximal activity, synthesis of alpha,alpha-trehalose | Pleurotus ostreatus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.004 | - |
vanadate | - |
Pleurotus ostreatus |