Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Escherichia coli | |
Mg2+ | - |
Escherichia coli |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli cells BL21-DE3-pLysS | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Escherichia coli | |
Ni2+ | - |
Escherichia coli | |
Zn2+ | - |
Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
biotinylated citronellyl-lipid I | in the presence of Mn2+ | Escherichia coli | |
0.037 | - |
biotinylated citronellyl-lipid I | in the presence of Mg2+ | Escherichia coli | |
0.044 | - |
biotinylated citronellyl-lipid I | in the absence of additional metal ions | Escherichia coli | |
0.045 | - |
UDP-N-acetylglucosamine | in the absence of additional metal ions | Escherichia coli | |
0.046 | - |
UDP-N-acetylglucosamine | in the presence of Mn2+ | Escherichia coli | |
0.058 | - |
UDP-N-acetylglucosamine | in the presence of Mg2+ | Escherichia coli |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | cytoplasmic fraction | Escherichia coli | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | activating compound | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Escherichia coli |
76000 | - |
gel filtration | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
wild type | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Storage Stability | Organism |
---|---|
4°C, stable for at least one month | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + biotinylated citronellyl-lipid I | - |
Escherichia coli | ? | - |
? | |
UDP-N-acetylglucosamine + citronellyl-lipid I | synthetic substrate, 55-carbon undecaprenol chain replaced by the 10-carbon chain of citronellol, biotin labeled substrate | Escherichia coli | UDP + N-acetylglucosamine-citronellyl-lipid I | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.123 | - |
biotinylated citronellyl-lipid I | in the absence of additional metal ions | Escherichia coli | |
0.267 | - |
biotinylated citronellyl-lipid I | in the presence of Mg2+ | Escherichia coli | |
0.317 | - |
biotinylated citronellyl-lipid I | in the presence of Mn2+ | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.3 | - |
Hepes buffer | Escherichia coli |