Literature summary for 2.4.1.22 extracted from

Navarre, C.; Smargiasso, N.; Duvivier, L.; Nader, J.; Far, J.; De Pauw, E.; Boutry, M.
N-Glycosylation of an IgG antibody secreted by Nicotiana tabacum BY-2 cells can be modulated through co-expression of human beta-1,4-galactosyltransferase (2017), Transgenic Res., 26, 375-384 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene B4GALT1, recombinant co-expression of a human IgG2 with a chimeric human GalT targeted to the cis- or trans-Golgi in Nicotiana tabacum strain BY-2 cell microsomes	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	carbohydrate characterization of an antibody (Lo-BM2) expressed in Nicotiana tabacum strain BY-2 cells shows that the purified Lo-BM2 displays N-glycan homogeneity with a high proportion (about 70%) of the complex GnGnXF glycoform. The stable co-expression of a human beta-1,4-galactosyltransferase targeted to different Golgi subcompartments alters Lo-BM2N-glycosylation and results in the production of an antibody that exhibits either hybrid structures containing a low abundance of the plant epitopes (alpha-1,3-fucose and beta-1,2-xylose), or a large amount of galactose-extended N-glycan structures. Targeting of GalT to different Golgi subcompartments. The sequence corresponding to the catalytic region (amino acid residues 123-397) of the human GalT precursor is fused via a SpeI restriction site to the first 77 amino acids from Nicotiana tabacum GNTI, corresponding to the CTS domain	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
Golgi apparatus	-	Homo sapiens	5794	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P15291	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-D-galactose + O-beta-D-xylosyl-[IgG antibody]	antibody (Lo-BM2) expressed in Nicotiana tabacum strain BY-2 cells	Homo sapiens	UDP + 4-beta-D-galactosyl-O-beta-D-xylosyl-[IgG antibody]	-	?

Synonyms

Synonyms	Comment	Organism
B4GALT1	-	Homo sapiens
beta-1,4-galactosyltransferase	-	Homo sapiens
GALT	-	Homo sapiens

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Release 2023.1 (January 2023)