Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cardiolipin | required for full activity, activates up to 10fold, 14-18 molecules of cardiolipin are bound to one molecule of enzyme | Streptococcus pyogenes |
Cloned (Comment) | Organism |
---|---|
expression as a soluble active protein comprising residues 1-703 | Pasteurella multocida |
overexpression in Escherichia coli, enzyme cannot be expressed as a soluble active protein | Streptococcus pyogenes |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cell membrane | most parts of the enzyme, except the transmembrane region, are cytoplasmic including the active site | Streptococcus pyogenes | - |
- |
additional information | probably cytoplasm or periplasm | Pasteurella multocida | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Streptococcus pyogenes | |
Mg2+ | required by class II enzyme | Pasteurella multocida |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
1 * 48000, without cardiolipin, radiation inactivation analysis | Streptococcus pyogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Streptococcus pyogenes | enzyme is responsible for hyaluronan biosynthesis, the hyaluronan capsule is an important, but not the only, virulence factor, physiological role of the enzyme | ? | - |
? | |
additional information | Pasteurella multocida | enzyme is responsible for hyaluronan biosynthesis, the hyaluronan capsule is an important, but not the only, virulence factor, physiological role of the enzyme | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pasteurella multocida | - |
avian pathogen, class II enzyme | - |
Streptococcus pyogenes | - |
human pathogen, single gene hasA in the has operon, class I enzyme | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli, native enzyme to homogeneity | Streptococcus pyogenes |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] | catalytic mechanism | Pasteurella multocida | |
UDP-N-acetyl-alpha-D-glucosamine + beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] = UDP + N-acetyl-beta-D-glucosaminyl-(1->4)-beta-D-glucuronosyl-(1->3)-N-acetyl-beta-D-glucosaminyl-(1->4)-[nascent hyaluronan] | catalytic mechanism, no covalent intermediates | Streptococcus pyogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is responsible for hyaluronan biosynthesis, the hyaluronan capsule is an important, but not the only, virulence factor, physiological role of the enzyme | Streptococcus pyogenes | ? | - |
? | |
additional information | enzyme is responsible for hyaluronan biosynthesis, the hyaluronan capsule is an important, but not the only, virulence factor, physiological role of the enzyme | Pasteurella multocida | ? | - |
? | |
additional information | enzyme acts processive, the enzyme is active as a complex with cardiolipin, a bacterial membrane lipid | Streptococcus pyogenes | ? | - |
? | |
additional information | enzyme is not processive, enzyme requires other proteins for hyaluronan translocation | Pasteurella multocida | ? | - |
? | |
UDP-D-glucosamine + UDP-D-glucuronate | - |
Streptococcus pyogenes | [beta-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | - |
? | |
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | addition of monosaccharides to the reducing end of the acceptor substrate, no binding and activity with exogenously added hyaluronan chains | Streptococcus pyogenes | [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | the product chain length can grow at the reducing end up to 40000 monosaccharides with a MW of over 8 million Da before it is released by the class I enzyme | ? | |
UDP-N-acetyl-D-glucosamine + UDP-D-glucuronate | chains growth at the non-reducing end, which is terminated by lack of substrate with a non-reducing end, active with exogenously added acceptors substrates | Pasteurella multocida | [beta-N-acetyl-D-glucosaminyl(1-4)beta-D-glucuronosyl(1-3)]n + UDP | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 48000, without cardiolipin, radiation inactivation analysis | Streptococcus pyogenes |
More | enzyme topology and organization, overview | Streptococcus pyogenes |
More | predicted enzyme topology and organization, overview | Pasteurella multocida |
Synonyms | Comment | Organism |
---|---|---|
HA synthase | - |
Streptococcus pyogenes |
HA synthase | - |
Pasteurella multocida |
More | the enzyme of Pasteurella multocida belongs to the group of class II hyaluronan synthases | Pasteurella multocida |
More | the enzyme of Streptococcus pyogenes belongs to the group of class I hyaluronan synthases | Streptococcus pyogenes |