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Literature summary for 2.4.1.198 extracted from
- Ras signaling activates glycosylphosphatidylinositol (GPI) anchor biosynthesis via the GPI-N-acetylglucosaminyltransferase (GPI-GnT) in Candida albicans (2018), J. Biol. Chem., 293, 12222-12238 .
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| CaRas1 | localizes to the cytoplasm or endoplasmic reticulum (ER) is sufficient for GPI-GnT activation. Mutational analysis, overview | Candida albicans | |
| additional information | Ras signaling enhances GPI-GnT activity | Candida albicans |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene GPI1, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, RT-PCR enzyme expression analysis | Candida albicans |
| gene GPI15, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, phenotype, RT-PCR enzyme expression analysis | Candida albicans |
| gene GPI19, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, RT-PCR enzyme expression analysis | Candida albicans |
| gene GPI2, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, phenotype, RT-PCR enzyme expression analysis | Candida albicans |
| gene GPI3, recombinant expression in the GPI-GnT heterozygous WY-ZY4 mutant strain, RT-PCR enzyme expression analysis | Candida albicans |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | analysis of enzyme-deficient WY-ZY4 strain (BWP17- Caras1/Caras2 mutant). CaGpi2 overexpression results in Hsp90 down-regulation, which is reflected in a decrease in transcript levels as well as in Hsp90 activity. Depletion of CaGpi2 can perhaps reduce the levels of CaRas1 available at the PM for signaling in the cAMP/PKA pathway for hyphal morphogenesis. Generation of revertant strains for all GPI-GnT subunits are created in their respective heterozygous strains | Candida albicans |
| additional information | analysis of enzyme-deficient WY-ZY4 strain (BWP17- Caras1/Caras2 mutant). Depletion of CaGpi2 can perhaps reduce the levels of CaRas1 available at the PM for signaling in the cAMP/PKA pathway for hyphal morphogenesis. Generation of revertant strains for all GPI-GnT subunits are created in their respective heterozygous strains | Candida albicans |
| additional information | analysis of enzyme-deficient WY-ZY4 strain (BWP17- Caras1/Caras2 mutant). Generation of revertant strains for all GPI-GnT subunits are created in their respective heterozygous strains | Candida albicans |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Candida albicans | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine + 1-phosphatidyl-1D-myo-inositol | Candida albicans | - |
UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | - |
? |  |
| UDP-N-acetyl-alpha-D-glucosamine + 1-phosphatidyl-1D-myo-inositol | Candida albicans ATCC MYA-2876 | - |
UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Candida albicans | - |
- |
- |
| Candida albicans | A0A1D8PHD4 | - |
- |
| Candida albicans | A0A1D8PKB8 | - |
- |
| Candida albicans | Q5A6L6 | - |
- |
| Candida albicans | Q5A9D6 | - |
- |
| Candida albicans ATCC MYA-2876 | - |
- |
- |
| Candida albicans ATCC MYA-2876 | A0A1D8PHD4 | - |
- |
| Candida albicans ATCC MYA-2876 | A0A1D8PKB8 | - |
- |
| Candida albicans ATCC MYA-2876 | Q5A6L6 | - |
- |
| Candida albicans ATCC MYA-2876 | Q5A9D6 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-glucosamine + 1-phosphatidyl-1D-myo-inositol | - |
Candida albicans | UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
| UDP-N-acetyl-alpha-D-glucosamine + 1-phosphatidyl-1D-myo-inositol | - |
Candida albicans ATCC MYA-2876 | UDP + 6-(N-acetyl-alpha-D-glucosaminyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| GPI-GnT | - |
Candida albicans |
| GPI-N-acetylglucosaminyltransferase | - |
Candida albicans |
| GPI1 | - |
Candida albicans |
| GPI15 | - |
Candida albicans |
| GPI19 | - |
Candida albicans |
| GPI2 | - |
Candida albicans |
| GPI3 | - |
Candida albicans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Candida albicans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| malfunction | in the CaGPI19 conditional null strain, isozyme CaGpi2 is overexpressed | Candida albicans |
| malfunction | the strain overexpressing CaGpi2 is hyperfilamentous and also heat-shock-sensitive, a phenotype typical of hyperactive Ras mutants. When Hsp90 levels are downregulated, due to CaGpi2 overexpression, the interaction of CaRas1 with Cyr1 is promoted at the cost of its interaction with Ira2. Thus, the filamentation pathway remains turned on even at 30°C, resulting in a hyperfilamentous phenotype | Candida albicans |
| metabolism | the ability of Candida albicans to switch between yeast to hyphal form is a property that is primarily associated with the invasion and virulence of this human pathogenic fungus. Several glycosylphosphatidylinositol (GPI)-anchored proteins are expressed only during hyphal morphogenesis. One of the major pathways that controls hyphal morphogenesis is the Ras signaling pathway. Cross-talk between GPI anchor biosynthesis and Ras signaling occurs in Candida albicans. The first step of GPI biosynthesis is activated by Ras in Candida albicans. Activation of Ras signaling is independent of the catalytic competence of GPI-GnT | Candida albicans |
| metabolism | the ability of Candida albicans to switch between yeast to hyphal form is a property that is primarily associated with the invasion and virulence of this human pathogenic fungus. Several glycosylphosphatidylinositol (GPI)-anchored proteins are expressed only during hyphal morphogenesis. One of the major pathways that controls hyphal morphogenesis is the Ras signaling pathway. Cross-talk between GPI anchor biosynthesis and Ras signaling occurs in Candida albicans. The first step of GPI biosynthesis is activated by Ras in Candida albicans. Activation of Ras signaling is independent of the catalytic competence of GPI-GnT. Possible interaction between Ras signaling and GPI-GnT in Candida albicans, detailed overview | Candida albicans |
| physiological function | of the two Candida albicans Ras proteins, CaRas1 alone activates GPI-GnT activity, and the activity is further stimulated by constitutively activated CaRas1. Of the six subunits of the GPI-N-acetylglucosaminyltransferase (GPI-GnT) that catalyze the first step of GPI biosynthesis, CaGpi2 is the key player involved in activating Ras signaling and hyphal morphogenesis. Activation of Ras signaling is independent of the catalytic competence of GPI-GnT | Candida albicans |
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