General Stability | Organism |
---|---|
freezing without glycerol, complete loss of activity | Secale cereale |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
luteolin | 0.02 mM, 50% inhibition | Secale cereale | |
UDP | strong | Secale cereale |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.009 | - |
Luteolin 7-O-diglucuronide | - |
Secale cereale | |
0.09 | - |
UDPglucuronic acid | - |
Secale cereale |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | not affected by Ca2+ or Mg2+ up to 0.5 and 0.75 mM | Secale cereale |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
gel filtration | Secale cereale |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | Secale cereale | involved in the sequential biosynthesis of luteolin triglucuronide | UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Secale cereale | - |
rye | - |
Purification (Comment) | Organism |
---|---|
Ultrogel AcA 44, Sephadex G-25, ultrafiltration, partial purification | Secale cereale |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Secale cereale | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.14 | - |
- |
Secale cereale |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, 3 months, 30-50% loss of activity | Secale cereale |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-galactose + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide] | 7.5% of activity with UDPglucuronic acid | Secale cereale | UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-galactoside | - |
? | |
UDP-glucose + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | 10% of activity with UDPglucuronic acid | Secale cereale | UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucoside | - |
? | |
UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | - |
Secale cereale | UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide | - |
ir | |
UDP-glucuronic acid + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | involved in the sequential biosynthesis of luteolin triglucuronide | Secale cereale | UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-glucuronide | - |
ir | |
UDP-glucuronic acid + luteolin-7-O-glucuronide | - |
Secale cereale | UDP + luteolin 7-O-beta-D-glucuronosyl-4'-O-glucuronide | - |
? | |
UDP-xylose + luteolin 7-O-[beta-D-glucuronosyl-(1->2)-beta-D-glucuronide] | 10% of activity with UDPglucuronic acid | Secale cereale | UDP + luteolin 7-O-[beta-D-glucuronosyl-1,2-beta-D-glucuronide]-4'-O-beta-D-xyloside | - |
? | |
UDPglucuronic acid + luteolin-7-O-glucuronide | 34% of activity with luteolin 7-O-diglucuronide | Secale cereale | UDP + luteolin 7-O-beta-D-glucuronosyl-4'-O-glucuronide | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Secale cereale |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Secale cereale |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | 50% of maximal activity at pH 6 and pH 9 | Secale cereale |