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Literature summary for 2.4.1.19 extracted from
- The cyclodextrin glycosyltransferase of Paenibacillus pabuli US132 strain: molecular characterization and overproduction of the recombinant enzyme (2008), J. Biomed. Biotechnol., 2008, 692573.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene cgtase, DNA and amino acid sequence determination and analysis, expression in Escherichia coli periplasmic fraction in different strains at low amounts, overview | Paenibacillus pabuli |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | the enzyme sequence contains a signal peptide of 34 residues | Paenibacillus pabuli | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 74300 | - |
x * 74300, sequence calculation | Paenibacillus pabuli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paenibacillus pabuli | B1VC16 | gene cgtase | - |
| Paenibacillus pabuli US132 | B1VC16 | gene cgtase | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 25fold from Escherichia coli, by heat treatment at 60°C, hydrophobic interaction chromatography and starch adsorption, overview | Paenibacillus pabuli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 5000 | - |
purified recombinant enzyme | Paenibacillus pabuli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the main amino acid residues of cyclization activity are Lys47, Tyr89, Asn94, Phe183, Asn193, Leu194, Tyr195, Asp196, Phe259, Phe283, and Asp371 | Paenibacillus pabuli | ? | - |
? |  |
| additional information | the main amino acid residues of cyclization activity are Lys47, Tyr89, Asn94, Phe183, Asn193, Leu194, Tyr195, Asp196, Phe259, Phe283, and Asp371 | Paenibacillus pabuli US132 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 74300, sequence calculation | Paenibacillus pabuli |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CGTase | - |
Paenibacillus pabuli |
| cyclodextrin glycosyltransferase | - |
Paenibacillus pabuli |
| More | CGTases belong to the glycoside hydrolase family 13, alpha-amylase family | Paenibacillus pabuli |
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