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Literature summary for 2.4.1.175 extracted from

  • Sobhany, M.; Kakuta, Y.; Sugiura, N.; Kimata, K.; Negishi, M.
    The chondroitin polymerase K4CP and the molecular mechanism of selective bindings of donor substrates to two active sites (2008), J. Biol. Chem., 283, 32328-32333.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.175

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.005895
-
 UDP-N-acetyl-alpha-D-glucosamine wild type enzyme, in 25 mM HEPES (pH 7.5), 100 mM NaCl, 20 mM MnCl2, 1 mM CaCl2, at 30°C Escherichia coli
0.005895
-
 UDP-N-acetyl-beta-D-galactosamine wild type enzyme, in 25 mM HEPES (pH 7.5), 100 mM NaCl, 20 mM MnCl2, 1 mM CaCl2, at 30°C Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli K4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
glutathione-Sepharose resin column chromatography Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no hydrolysis activity is observed with UDP-N-glucuronic acid Escherichia coli ?
-
 ?
additional information no hydrolysis activity is observed with UDP-N-glucuronic acid Escherichia coli K4 ?
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
-
 Escherichia coli UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide
-
 Escherichia coli K4 UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + chondroitin
-
 Escherichia coli ?
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + chondroitin
-
 Escherichia coli K4 ?
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + chondroitin sulfate oligosaccharide weak activity Escherichia coli UDP + N-acetyl-beta-D-glucosaminyl chondroitin sulfate oligosaccharide
-
 ?
UDP-N-acetyl-alpha-D-glucosamine + chondroitin sulfate oligosaccharide weak activity Escherichia coli K4 UDP + N-acetyl-beta-D-glucosaminyl chondroitin sulfate oligosaccharide
-
 ?
UDP-N-acetyl-beta-D-galactosamine + chondroitin
-
 Escherichia coli ?
-
 ?
UDP-N-acetyl-beta-D-galactosamine + chondroitin
-
 Escherichia coli K4 ?
-
 ?

Synonyms

Synonyms Comment Organism
chondroitin polymerase
-
 Escherichia coli
K4CP
-
 Escherichia coli