Cloned (Comment) | Organism |
---|---|
gene expression profiling of cells is conducted by DNA microarray analysis | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | MGAT5 gene knockout in HT-29 cells using CRISPR/Cas9 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | - |
Homo sapiens | 5794 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] | Homo sapiens | - |
UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[TIMP-1] | Homo sapiens | - |
UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[TIMP-1] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q09328 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colonic cancer cell | - |
Homo sapiens | - |
HT-29 cell | - |
Homo sapiens | - |
LoVo cell | - |
Homo sapiens | - |
additional information | transcriptome analysis. Overexpression of the MGAT5 gene has been found in various tumor cells and tissues | Homo sapiens | - |
SW-480 cell | - |
Homo sapiens | - |
WiDr cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] | - |
Homo sapiens | UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] | - |
? | |
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[TIMP-1] | - |
Homo sapiens | UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[TIMP-1] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GnT-V | - |
Homo sapiens |
Mgat5 | - |
Homo sapiens |
N-acetylglucosaminyltransferase V | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | overexpression of N-acetylglucosaminyltransferase V protects detached cancer cells from apoptotic death, and suppression or knockout of the gene sensitized cancer cells to the apoptotic death. The gene overexpression also stimulates anchorage-dependent as well as anchorage-independent colony formation of cancer cells following anoikis stress treatments | Homo sapiens |
physiological function | N-acetylglucosaminyltransferase V (GnT-V) is a Golgi-located enzyme that catalyzes the branching of the beta1,6-N-acetylglucosamine side chain to the core mannosyl residue of N-linked glycan. N-acetylglucosaminyltransferase V confers anoikis resistance to colon cancer cells during hematogenous metastasis. This resistance is abolished by the lectin from Sambucus sieboldiana, treatment with the lectin significantly sensitizes anoikis-induced cancer cell deaths in vitro as well as in vivo. Anoikis is a form of anchorage-dependent apoptosis, and cancer cells adopt anokis-resistance molecular machinery to conduct metastasis. The MGAT5 gene is a key modulator of anoikis resistance, MGAT5-stimulated anoikis resistance is validated in clinical specimens by correlating the MGAT5 mRNA levels to the cancer stage. MGAT5 potentiates anchorage-dependent and -independent growth following anoikis stress. Glycan profiling identifies unique N-glycan structural features involved in anoikis resistance | Homo sapiens |