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Literature summary for 2.3.3.17 extracted from

  • Petersen, A.; Hansen, L.G.; Mirza, N.; Crocoll, C.; Mirza, O.; Halkier, B.A.
    Changing substrate specificity and iteration of amino acid chain elongation in glucosinolate biosynthesis through targeted mutagenesis of Arabidopsis methylthioalkylmalate synthase 1 (2019), Biosci. Rep., 39, BSR20190446 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.3.17

Cloned(Commentary)

Cloned (Comment) Organism
fifteen MAM1 variants are characterised by as well as the native MAM1 heterologous expression in Escherichia coli NEB10B Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
A290G the strain harbouring the MAM1 variant produces more thrice-elongated methionine than the native MAM1 Arabidopsis thaliana
A290S unchanged composition of chain-elongated amino acids. The strain harbouring the MAM1 variant produces more thrice-elongated methionine than the native MAM1 Arabidopsis thaliana
A290T in the strain harbouring the MAM1 variant, SSU3 protein level is higher than in the strain harbouring wild-type MAM1. No chain-elongated amino acid products are found Arabidopsis thaliana
A290T the strain harbouring the MAM1 variant does not produce any of the chain-elongated amino acid products Arabidopsis thaliana
G259A in the strain harbouring the MAM1 variant, SSU3 protein level is higher than in the strain harbouring wild-type MAM1. No chain-elongated amino acid products are found Arabidopsis thaliana
G259A the strain harbouring the MAM1 variant does not produce amino acids elongated more than twice Arabidopsis thaliana
G259A the strain harbouring the MAM1 variant does not produce any of the chain-elongated amino acid products Arabidopsis thaliana
T257C the strain harbouring the MAM1 variant produces more thrice-elongated methionine than the native MAM1 Arabidopsis thaliana
T257D in the strain harbouring the MAM1 variant, SSU3 protein level is higher than in the strain harbouring wild-type MAM1. The strain harbouring the MAM1 variant produces more thrice-elongated methionine than the native MAM1 Arabidopsis thaliana
T257G the strain harbouring the MAM1 variant does not produce amino acids elongated more than twice Arabidopsis thaliana
T257G/G259A the strain harbouring the MAM1 variant produces tetra-elongated methionine Arabidopsis thaliana
T257G/G259A/A290G the strain harbouring the MAM1 variant produces tetra-elongated methionine. Unchanged composition of chain-elongated amino acids, although the production is dramatically reduced Arabidopsis thaliana
T257G/G259A/L186M the strain harbouring the MAM1 variant produces tetra-elongated methionine Arabidopsis thaliana
T257N T257S shows nearly 3fold higher L-homophenylalanine production than the native MAM1. In the strain harbouring the MAM1 variant, SSU3 protein level is higher than in the strain harbouring wild-type MAM1. Low levels of chain-elongated amino acid products are found Arabidopsis thaliana
T257S the strain harbouring the MAM1 variant shows higher MAM1 levels, although this does not result in correspondingly higher levels of chain-elongated products Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q9FG67 ecotype Col-0
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Synonyms

Synonyms Comment Organism
MAM1
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 Arabidopsis thaliana
methylthioalkylmalate synthase 1
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 Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism the enzyme catalyses the committing step of amino acid chain elongation in glucosinolate biosynthesis and plays an important role in determining the glucosinolate composition of Brassicaceae species, including Arabidopsis thaliana. MAM1 is responsible for homophenylalanine production Arabidopsis thaliana