Activating Compound | Comment | Organism | Structure |
---|---|---|---|
AMP | CS II, counteracted by NADH | Pseudomonas sp. |
General Stability | Organism |
---|---|
CS II much more stable than CS I | Pseudomonas sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis(2-nitrobenzoate) | inactivation, half-lifes at 0.4 mM: 2.8 min for CS 1, 50 min for CS II | Pseudomonas sp. | |
ADP | CS II insensitive, CS I inhibited competitively with acetyl-CoA, noncompetitively with oxaloacetate | Pseudomonas sp. | |
AMP | CS II insensitive, CS I inhibited competitively with acetyl-CoA, noncompetitively with oxaloacetate | Pseudomonas sp. | |
ATP | CS II insensitive, CS I inhibited | Pseudomonas sp. | |
KCl | CS I inhibited competitively with acetyl-CoA, noncompetitively with oxaloacetate | Pseudomonas sp. | |
NADH | CS II inhibited, CS II insensitive; weak inhibition, reversible by AMP, hinders the activation by AMP, CS II | Pseudomonas sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
oxaloacetate | CS I | Pseudomonas sp. | |
0.035 | - |
acetyl-CoA | CS I | Pseudomonas sp. | |
0.05 | - |
oxaloacetate | CS II | Pseudomonas sp. | |
0.45 | - |
acetyl-CoA | CS II | Pseudomonas sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
KCl | - |
Pseudomonas sp. | |
phosphate | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
2 * 53000, SDS-PAGE, CS I, reassociation to CS II possible | Pseudomonas sp. |
53000 | - |
6 * 53000, SDS-PAGE, CS II | Pseudomonas sp. |
100000 | - |
gel filtration | Pseudomonas sp. |
100000 | - |
CS I | Pseudomonas sp. |
300000 | - |
CS II, gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
marine, 2 enzyme forms with different molecular weight, kinetic and regulatory properties, dissociated CS I /native CS II | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
102.7 | - |
purified enzyme | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
4°C, Tris-HCl buffer, pH 7.6, 0.2 M KCl, 20% loss of activity after 1 month, only CS II | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + oxaloacetate + H2O | - |
Pseudomonas sp. | citrate + CoA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 53000, SDS-PAGE, CS I, reassociation to CS II possible | Pseudomonas sp. |
hexamer | 6 * 53000, SDS-PAGE, CS II | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
enzyme assay at | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
enzyme assay at | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
AMP | only CS II, counteracted by NADP | Pseudomonas sp. |