BRENDA - Enzyme Database
show all sequences of 2.3.1.B5

Polyhydroxybutyrate biosynthesis in Caulobacter crescentus: molecular characterization of the polyhydroxybutyrate synthase

Qi, Q.; Rehm, B.H.; Microbiology 147, 3353-3358 (2001)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
the phaC coding region is subcloned into vector pBBR1-JO2 under lac promoter control. The resulting plasmid, pQQ4, mediates PHB accumulation in the mutant Ralstonia eutropha PHBN4 and recombinant Escherichia coli JM109(pBHR69)
Caulobacter vibrioides
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
73000
-
x * 73000, SDS-PAGE
Caulobacter vibrioides
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Caulobacter vibrioides
Q9F4K5
-
-
Subunits
Subunits
Commentary
Organism
?
x * 73000, SDS-PAGE
Caulobacter vibrioides
Cloned(Commentary) (protein specific)
Commentary
Organism
the phaC coding region is subcloned into vector pBBR1-JO2 under lac promoter control. The resulting plasmid, pQQ4, mediates PHB accumulation in the mutant Ralstonia eutropha PHBN4 and recombinant Escherichia coli JM109(pBHR69)
Caulobacter vibrioides
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
73000
-
x * 73000, SDS-PAGE
Caulobacter vibrioides
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 73000, SDS-PAGE
Caulobacter vibrioides
Other publictions for EC 2.3.1.B5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735919
Klask
-
Heterologous expression of var ...
Rhodospirillum rubrum
Chem. Biochem. Eng. Q.
29
75-85
2015
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1
1
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737152
Hyakutake
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Carboxy-terminal modification ...
Bacillus cereus, Bacillus cereus YB-4
Polym. Degrad. Stab.
117
90-96
2015
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1
1
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735496
Hyakutake
Alcoholytic cleavage of polyhy ...
Bacillus cereus, Bacillus cereus YB-4
Appl. Environ. Microbiol.
80
1421-1429
2014
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1
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736277
Gowda
-
Poly(3)hydroxybutyrate (PHB) p ...
Bacillus thuringiensis, Bacillus thuringiensis IAM 12077
Int. J. Pharm. Bio Sci.
4
B794-B802
2013
1
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719032
Tomizawa
Molecular weight change of pol ...
Bacillus cereus, Bacillus cereus YB-4, Bacillus megaterium, Bacillus megaterium NBRC15308
Biomacromolecules
12
2660-2666
2011
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2
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2
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6
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4
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10
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10
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719746
Tan
Cloning and characterization o ...
Pseudomonas sp., Pseudomonas sp. USM4-55
J. Basic Microbiol.
50
179-189
2010
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1
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721109
Kek
-
Heterologous expression of Cup ...
Cupriavidus sp., Cupriavidus sp. USMAA2-4
World J. Microbiol. Biotechnol.
26
1595-1603
2010
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1
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684934
Dai
Poly-3-hydroxybutyrate synthas ...
Escherichia coli
Biochem. Biophys. Res. Commun.
374
485-489
2008
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688122
Sharma
Studies on poly-beta-hydroxybu ...
Desmonostoc muscorum
J. Gen. Appl. Microbiol.
52
209-214
2006
1
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684663
Hezayen
Biochemical and enzymological ...
Halopiger aswanensis, Halopiger aswanensis DSM 13151
Arch. Biochem. Biophys.
403
284-291
2002
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688851
Qi
Polyhydroxybutyrate biosynthes ...
Caulobacter vibrioides
Microbiology
147
3353-3358
2001
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689122
Sim
PHA synthase activity controls ...
Cupriavidus necator
Nat. Biotechnol.
15
63-67
1997
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687271
Wodzinska
-
Polyhydroxybutyrate synthase: ...
Cupriavidus necator
J. Am. Chem. Soc.
118
6319-6320
1996
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