Any feedback?
Literature summary for 2.3.1.B46 extracted from
- Caenorhabditis elegans mboa-7, a member of the MBOAT family, is required for selective incorporation of polyunsaturated fatty acids into phosphatidylinositol (2008), Mol. Biol. Cell, 19, 1174-1184 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene mboa-7, DNA and amino acid sequence determination and analysis, sequence comparisons | Caenorhabditis elegans |
| gene Mboat7, cloning from a liver DNA library, DNA and amino acid sequence determination and analysis, sequence comparisons | Mus musculus |
| gene MBOAT7, cloning from HeLa cells, DNA and amino acid sequence determination and analysis, sequence comparisons, quantitative real-time reverse transcription PCR enzyme expression analysis | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of mboa-7 mutants, the following mutations are used: fat-1(wa9), fat-3(wa22), fat-4(wa14), eri-1(mg366), and mboa-7(gk399). mboa-7(gk399) mutation is backcrossed at five times, phenotype, detailed overview. fat-4 fat-1 mutants, which do not produce AA(20:4n-6) and n-3 PUFAs such as EPA(20:5n-3), are viable and fertile, but they exhibited slow growth at 15°C. Dietary supplementation with arachidonic acid (AA) or eicosapentaenoic acid (EPA) leads to incorporation of these PUFAs into the phospholipid fractions of fat-4 fat-1 mutants, and it rescues the growth defects of this strain. Fatty acid composition of wild-type and mboa-7 mutants, overview | Caenorhabditis elegans |
| additional information | in cells transfected with hmboa-7 siRNA duplex, h-mboa-7 mRNA levels are reduced to about 30% of the level in control siRNA-transfected cells at 72 h after the transfection. Incorporation of [14C]AA into cellular PI is specifically reduced from 13.1% of the total radioactivity in control cells to 6.7% in the siRNA-transfected cells. Incorporation of [14C]EPA into PI is also reduced similar to that of [14C]AA in siRNA-transfected cells | Homo sapiens |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | membrane-bound | Homo sapiens | 16020 | - |
| membrane | membrane-bound | Mus musculus | 16020 | - |
| membrane | membrane-bound | Caenorhabditis elegans | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidonoyl-CoA + 1-acyl-lysophosphatidylinositol | Homo sapiens | - |
CoA + 1-acyl-2-arachidonoyl-lysophosphatidylinositol | - |
? |  |
| arachidonoyl-CoA + 1-acyl-lysophosphatidylinositol | Mus musculus | - |
CoA + 1-acyl-2-arachidonoyl-lysophosphatidylinositol | - |
? | |
| arachidonoyl-CoA + 1-acyl-lysophosphatidylinositol | Caenorhabditis elegans | - |
CoA + 1-acyl-2-arachidonoyl-lysophosphatidylinositol | - |
? | |
| arachidonoyl-CoA + 1-acyl-lysophosphatidylinositol | Caenorhabditis elegans Bristol N2 | - |
CoA + 1-acyl-2-arachidonoyl-lysophosphatidylinositol | - |
? | |
| eicosapentaenoyl-CoA + 1-acyl-lysophosphatidylinositol | Homo sapiens | - |
CoA + 1-acyl-2-eicosapentaenoyl-lysophosphatidylinositol | - |
? | |
| eicosapentaenoyl-CoA + 1-acyl-lysophosphatidylinositol | Mus musculus | - |
CoA + 1-acyl-2-eicosapentaenoyl-lysophosphatidylinositol | - |
? | |
| eicosapentaenoyl-CoA + 1-acyl-lysophosphatidylinositol | Caenorhabditis elegans | - |
CoA + 1-acyl-2-eicosapentaenoyl-lysophosphatidylinositol | - |
? | |
| eicosapentaenoyl-CoA + 1-acyl-lysophosphatidylinositol | Caenorhabditis elegans Bristol N2 | - |
CoA + 1-acyl-2-eicosapentaenoyl-lysophosphatidylinositol | - |
? | |
| additional information | Mus musculus | LPLAT 7 prefers 20:4-CoA and 20:5-CoA as donors | ? | - |
- |
|
| additional information | Caenorhabditis elegans | LPLAT 7 prefers 20:4-CoA and 20:5-CoA as donors. Analysis of in vivo incorporation of exogenous fatty acids into Caenorhabditis elegans | ? | - |
- |
|
| additional information | Homo sapiens | LPLAT 7 prefers 20:4-CoA and 20:5-CoA as donors. Analysis of in vivo incorporation of exogenous fatty acids into HeLa cells | ? | - |
- |
|
| additional information | Caenorhabditis elegans Bristol N2 | LPLAT 7 prefers 20:4-CoA and 20:5-CoA as donors. Analysis of in vivo incorporation of exogenous fatty acids into Caenorhabditis elegans | ? | - |
- |
|
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Caenorhabditis elegans | Q19468 | - |
- |
| Caenorhabditis elegans Bristol N2 | Q19468 | - |
- |
| Homo sapiens | Q96N66 | - |
- |
| Mus musculus | Q8CHK3 | - |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| breast cancer cell | - |
Mus musculus | - |
| HeLa cell | - |
Homo sapiens | - |
| liver | - |
Mus musculus | - |
| additional information | Escherichia coli OP50, a bacterium that does not synthesize or require polyunsaturated fatty acids, is used as the sole food source | Caenorhabditis elegans | - |
| additional information | the enzyme is reported to be upregulated in metastatic breast and bladder carcinomas | Mus musculus | - |
| urinary bladder cancer cell | - |
Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| arachidonoyl-CoA + 1-acyl-lysophosphatidylinositol | - |
Homo sapiens | CoA + 1-acyl-2-arachidonoyl-lysophosphatidylinositol | - |
? | |
| arachidonoyl-CoA + 1-acyl-lysophosphatidylinositol | - |
Mus musculus | CoA + 1-acyl-2-arachidonoyl-lysophosphatidylinositol | - |
? | |
| arachidonoyl-CoA + 1-acyl-lysophosphatidylinositol | - |
Caenorhabditis elegans | CoA + 1-acyl-2-arachidonoyl-lysophosphatidylinositol | - |
? | |
| arachidonoyl-CoA + 1-acyl-lysophosphatidylinositol | - |
Caenorhabditis elegans Bristol N2 | CoA + 1-acyl-2-arachidonoyl-lysophosphatidylinositol | - |
? | |
| eicosapentaenoyl-CoA + 1-acyl-lysophosphatidylinositol | - |
Homo sapiens | CoA + 1-acyl-2-eicosapentaenoyl-lysophosphatidylinositol | - |
? | |
| eicosapentaenoyl-CoA + 1-acyl-lysophosphatidylinositol | - |
Mus musculus | CoA + 1-acyl-2-eicosapentaenoyl-lysophosphatidylinositol | - |
? | |
| eicosapentaenoyl-CoA + 1-acyl-lysophosphatidylinositol | - |
Caenorhabditis elegans | CoA + 1-acyl-2-eicosapentaenoyl-lysophosphatidylinositol | - |
? | |
| eicosapentaenoyl-CoA + 1-acyl-lysophosphatidylinositol | - |
Caenorhabditis elegans Bristol N2 | CoA + 1-acyl-2-eicosapentaenoyl-lysophosphatidylinositol | - |
? | |
| additional information | LPLAT 7 prefers 20:4-CoA and 20:5-CoA as donors | Homo sapiens | ? | - |
- |
|
| additional information | LPLAT 7 prefers 20:4-CoA and 20:5-CoA as donors | Mus musculus | ? | - |
- |
|
| additional information | LPLAT 7 prefers 20:4-CoA and 20:5-CoA as donors | Caenorhabditis elegans | ? | - |
- |
|
| additional information | LPLAT 7 prefers 20:4-CoA and 20:5-CoA as donors. Analysis of in vivo incorporation of exogenous fatty acids into Caenorhabditis elegans | Caenorhabditis elegans | ? | - |
- |
|
| additional information | LPLAT 7 prefers 20:4-CoA and 20:5-CoA as donors. Analysis of in vivo incorporation of exogenous fatty acids into HeLa cells | Homo sapiens | ? | - |
- |
|
| additional information | LPLAT 7 prefers 20:4-CoA and 20:5-CoA as donors | Caenorhabditis elegans Bristol N2 | ? | - |
- |
|
| additional information | LPLAT 7 prefers 20:4-CoA and 20:5-CoA as donors. Analysis of in vivo incorporation of exogenous fatty acids into Caenorhabditis elegans | Caenorhabditis elegans Bristol N2 | ? | - |
- |
|
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BB1 | - |
Homo sapiens |
| LPIAT | - |
Homo sapiens |
| LPIAT | - |
Mus musculus |
| LPIAT | - |
Caenorhabditis elegans |
| LPIAT1 | - |
Homo sapiens |
| LPIAT1 | - |
Mus musculus |
| LPIAT1 | - |
Caenorhabditis elegans |
| LPLAT 7 | - |
Homo sapiens |
| LPLAT 7 | - |
Mus musculus |
| LPLAT 7 | - |
Caenorhabditis elegans |
| lysophosphatidylinositol-acyltransferase-1 | - |
Homo sapiens |
| lysophosphatidylinositol-acyltransferase-1 | - |
Mus musculus |
| lysophosphatidylinositol-acyltransferase-1 | - |
Caenorhabditis elegans |
| lysophospholipid acyltransferase 7 | - |
Homo sapiens |
| lysophospholipid acyltransferase 7 | - |
Mus musculus |
| lysophospholipid acyltransferase 7 | - |
Caenorhabditis elegans |
| LysoPI acyltransferase | - |
Homo sapiens |
| LysoPI acyltransferase | - |
Mus musculus |
| LysoPI acyltransferase | - |
Caenorhabditis elegans |
| mboa-7 | - |
Homo sapiens |
| mboa-7 | - |
Mus musculus |
| mboa-7 | - |
Caenorhabditis elegans |
| MBOAT7 | - |
Caenorhabditis elegans |
| MBOAT7 | - |
Homo sapiens |
| MBOAT7 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | - |
assay at | Homo sapiens |
| 20 | - |
assay at | Mus musculus |
| 20 | - |
assay at | Caenorhabditis elegans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
assay at | Homo sapiens |
| 7 | - |
assay at | Mus musculus |
| 7 | - |
assay at | Caenorhabditis elegans |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the MBOAT family | Homo sapiens |
| evolution | the enzyme belongs to the MBOAT family | Mus musculus |
| evolution | the enzyme belongs to the MBOAT family | Caenorhabditis elegans |
| malfunction | incorporation of exogenous PUFA into PI of the living worms and LPIAT activity in the microsomes are greatly reduced in mboa-7 mutants. Furthermore, the membrane fractions of transgenic worms expressing recombinant MBOA-7 and its human homologue exhibit remarkably increased LPIAT activity. Gene mboa-7 mutants have significantly lower eicosapentaenoic acid (EPA) levels in phosphatidylinositol (PI), and they exhibit larval arrest and egg-laying defects. Fatty acid composition of wild-type and mboa-7 mutants, overview | Caenorhabditis elegans |
| physiological function | the enzyme is required for selective incorporation of polyunsaturated fatty acids (PUFAs) into phosphatidylinositol. Phosphatidylinositol (PI) is a component of membrane phospholipids, and it functions both as a signaling molecule and as a compartment-specific localization signal in the form of polyphosphoinositides. Arachidonic acid (AA) is the predominant fatty acid in the sn-2 position of PI in mammals. LysoPI acyltransferase (LPIAT) is thought to catalyze formation of AA-containing PI | Homo sapiens |
| physiological function | the enzyme is required for selective incorporation of polyunsaturated fatty acids (PUFAs) into phosphatidylinositol. Phosphatidylinositol (PI) is a component of membrane phospholipids, and it functions both as a signaling molecule and as a compartment-specific localization signal in the form of polyphosphoinositides. Arachidonic acid (AA) is the predominant fatty acid in the sn-2 position of PI in mammals. LysoPI acyltransferase (LPIAT) is thought to catalyze formation of AA-containing PI | Mus musculus |
| physiological function | the enzyme is required for selective incorporation of polyunsaturated fatty acids (PUFAs) into phosphatidylinositol. Phosphatidylinositol (PI) is a component of membrane phospholipids, and it functions both as a signaling molecule and as a compartment-specific localization signal in the form of polyphosphoinositides. Arachidonic acid (AA) is the predominant fatty acid in the sn-2 position of PI in mammals. LysoPI acyltransferase (LPIAT) is thought to catalyze formation of AA-containing PI. In Caenorhabditis elegans, eicosapentaenoic acid (EPA) instead of AA is the predominant fatty acid in PI | Caenorhabditis elegans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
