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Literature summary for 2.3.1.97 extracted from

  • Dian, C.; Perez-Dorado, I.; Riviere, F.; Asensio, T.; Legrand, P.; Ritzefeld, M.; Shen, M.; Cota, E.; Meinnel, T.; Tate, E.; Giglione, C.
    High-resolution snapshots of human N-myristoyltransferase in action illuminate a mechanism promoting N-terminal Lys and Gly myristoylation (2020), Nat. Commun., 11, 1132 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.97

Crystallization (Commentary)

Crystallization (Comment) Organism
NMT1 structures in complex with reactive cognate lipid and peptide substrates Homo sapiens

Protein Variants

Protein Variants Comment Organism
K107E/K252E substitution does not alter catalytic efficiency with respect to the wild-type Homo sapiens
V181A 78% of wild-type activity Homo sapiens
V181L 26% of wild-type activity Homo sapiens
Y180A 0.9% of wild-type activity Homo sapiens
Y180F/N246A substitution does not alter catalytic efficiency with respect to the wild-type Homo sapiens
Y180P less than 0.01% of wild-type activity Homo sapiens
Y192A 21% of wild-type activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 myristoyl-CoA wild-type, pH 8.0, 30°C Homo sapiens
0.02
-
 myristoyl-CoA mutant V181A, pH 8.0, 30°C Homo sapiens
0.033
-
 myristoyl-CoA mutant Y180F/N246A, pH 8.0, 30°C Homo sapiens
0.051
-
 myristoyl-CoA mutant K107E/K252E, pH 8.0, 30°C Homo sapiens
0.054
-
 myristoyl-CoA mutant V181L, pH 8.0, 30°C Homo sapiens
0.187
-
 myristoyl-CoA mutant Y192A, pH 8.0, 30°C Homo sapiens
0.765
-
 myristoyl-CoA mutant Y180A, pH 8.0, 30°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P30419
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information NMT acts both as N-terminal lysine and glycine myristoyltransferase Homo sapiens ?
-
-
myristoyl-CoA + Gly-Cys-Ser-Val-Ser-Lys-Lys-Lys
-
 Homo sapiens CoA + N-myristoyl-Gly-Cys-Ser-Val-Ser-Lys-Lys-Lys
-
 ?

Synonyms

Synonyms Comment Organism
NMT1
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.16
-
 myristoyl-CoA mutant Y180A, pH 8.0, 30°C Homo sapiens
0.33
-
 myristoyl-CoA mutant V181L, pH 8.0, 30°C Homo sapiens
0.36
-
 myristoyl-CoA mutant V181A, pH 8.0, 30°C Homo sapiens
0.41
-
 myristoyl-CoA wild-type, pH 8.0, 30°C Homo sapiens
0.68
-
 myristoyl-CoA mutant Y180F/N246A, pH 8.0, 30°C Homo sapiens
0.93
-
 myristoyl-CoA mutant Y192A, pH 8.0, 30°C Homo sapiens
1.33
-
 myristoyl-CoA mutant K107E/K252E, pH 8.0, 30°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.206
-
 myristoyl-CoA mutant Y180A, pH 8.0, 30°C Homo sapiens
4.96
-
 myristoyl-CoA mutant Y192A, pH 8.0, 30°C Homo sapiens
6.17
-
 myristoyl-CoA mutant V181L, pH 8.0, 30°C Homo sapiens
18.54
-
 myristoyl-CoA mutant V181A, pH 8.0, 30°C Homo sapiens
20.92
-
 myristoyl-CoA mutant Y180F/N246A, pH 8.0, 30°C Homo sapiens
23.5
-
 myristoyl-CoA wild-type, pH 8.0, 30°C Homo sapiens
25.85
-
 myristoyl-CoA mutant K107E/K252E, pH 8.0, 30°C Homo sapiens