Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Grewia asiatica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Grewia asiatica | - |
- |
- |
Purification (Comment) | Organism |
---|---|
glutathione-Sepharose column chromatography | Grewia asiatica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | - |
Grewia asiatica | - |
leaf | - |
Grewia asiatica | - |
petal | - |
Grewia asiatica | - |
root | highest expression of isoform CHS1 | Grewia asiatica | - |
stamen | - |
Grewia asiatica | - |
stem | highest expression of isoform CHS2 | Grewia asiatica | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + 4-coumaroyl-CoA | the reaction has highest efficiency with isoform CHS1. 4-coumaroyl-CoA is the best substrate for isoform CHS1 | Grewia asiatica | 4 CoA + naringenin chalcone + 3 CO2 | - |
? | |
malonyl-CoA + acetyl-CoA | the reaction has highest efficiency with isoform CHS2 | Grewia asiatica | ? | - |
? | |
malonyl-CoA + butyryl-CoA | the reaction has highest efficiency with isoform CHS2 | Grewia asiatica | ? | - |
? | |
malonyl-CoA + hexanoyl-CoA | the reaction has highest efficiency with isoform CHS2. Hexanoyl-CoA is the best substrate for isoform CHS2 | Grewia asiatica | ? | - |
? | |
malonyl-CoA + octanoyl-CoA | the reaction has highest efficiency with isoform CHS2 | Grewia asiatica | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 43000, SDS-PAGE | Grewia asiatica |
Synonyms | Comment | Organism |
---|---|---|
Chs1 | isoform | Grewia asiatica |
Chs2 | isoform | Grewia asiatica |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Grewia asiatica | isoform CHS2, calculated from amino acid sequence | - |
7.6 |
Grewia asiatica | isoform CHS1, calculated from amino acid sequence | - |
10.1 |
Organism | Comment | Expression |
---|---|---|
Grewia asiatica | the enzyme expression increases during flowering | up |