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Literature summary for 2.3.1.74 extracted from

  • Christensen, A.B.; Gregersen, P.L.; Schroder, J.; Collinge, D.B.
    A chalcone synthase with an unusual substrate preference is expressed in barley leaves in response to UV light and pathogen attack (1998), Plant Mol. Biol., 37, 849-857.
    View publication on PubMed
Search for more literature for 2.3.1.74

Cloned(Commentary)

Cloned (Comment) Organism
wild type enzyme and HvCHS2 with different substrate requirements Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
 feruloyl-CoA
-
 Hordeum vulgare
0.002
-
 4-coumaroyl-CoA
-
 Hordeum vulgare

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43000
-
 wild type enzyme migrates at 43000 Da, HvCHS2 migrated at a slightly lower molecular mass Hordeum vulgare

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
 barley, inoculated with fungus blumeria graminis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
caffeoyl-CoA + 3 malonyl-CoA wild type enzyme uses cinnamoyl-CoA and 4-coumaroyl-CoA at comparable rates whereas feruloyl-CoA is a poor substrate, HvCHS2 converts feruloyl-CoA and caffeoyl-CoA at the highest rate whereas cinnamoyl-CoA is a poor substrate Hordeum vulgare eriodictyol + 4 CoA + 3 CO2
-
 ?
cinnamoyl-CoA + malonyl-CoA wild type enzyme uses cinnamoyl-CoA and 4-coumaroyl-CoA at comparable rates whereas feruloyl-CoA is a poor substrate, HvCHS2 converts feruloyl-CoA and caffeoyl-CoA at the highest rate whereas cinnamoyl-CoA is a poor substrate Hordeum vulgare 5,7-dihydroxyflavanone + CoA + CO2
-
 ?
ferulyl-CoA + malonyl-CoA wild type enzyme uses cinnamoyl-CoA and 4-coumaroyl-CoA at comparable rates whereas feruloyl-CoA is a poor substrate, HvCHS2 converts feruloyl-CoA and caffeoyl-CoA at the highest rate whereas cinnamoyl-CoA is a poor substrate Hordeum vulgare homoeriodictyol + CoA + CO2
-
 ?
malonyl-CoA + 4-coumaroyl-CoA wild type enzyme uses cinnamoyl-CoA and 4-coumaroyl-CoA at comparable rates whereas feruloyl-CoA is a poor substrate, HvCHS2 converts feruloyl-CoA and caffeoyl-CoA at the highest rate whereas cinnamoyl-CoA is a poor substrate Hordeum vulgare naringenin chalcone + CoA + CO2
-
 ?