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Literature summary for 2.3.1.74 extracted from
- Chalcone synthase from cell suspension cultures of Daucus carota L (1985), Arch. Biochem. Biophys., 240, 265-272.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | also leads to increased formation of by-products | Daucus carota |  |
| bovine serum albumin | - |
Daucus carota | |
| chlorogenic acid | increases eriodictyol formation by 20% | Daucus carota | |
General Stability
| General Stability | Organism |
|---|---|
| withdrawal of 2-mercaptoethanol after ammonium sulfate precipitation leads to increased stability during further purification | Daucus carota |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-coumaroyl-CoA | above 0.01 mM | Daucus carota | |
| cerulenin | - |
Daucus carota | |
| eriodictyol | - |
Daucus carota | |
| eriodictyol chalcone | - |
Daucus carota | |
| malonyl-CoA | substrate inhibition above 0.05 mM | Daucus carota | |
| naringenin | 50% inhibition of naringenin formation and total inhibition of eriodictyol formation | Daucus carota | |
| naringenin chalcone | 50% inhibition of naringenin formation and total inhibition of eriodictyol formation | Daucus carota | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0006 | - |
4-coumaroyl-CoA | - |
Daucus carota | |
| 0.003 | - |
malonyl-CoA | - |
Daucus carota | |
| 0.0077 | - |
caffeoyl-CoA | - |
Daucus carota | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumaroyl-CoA + 3 malonyl-CoA | Daucus carota | - |
naringenin chalcone + 4 CoA + 3 CO2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Daucus carota | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Daucus carota |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell suspension culture | - |
Daucus carota | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.162 | - |
- |
Daucus carota |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, decrease of activity in solutions below 1 mg/ml | Daucus carota |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-coumaroyl-CoA + 3 malonyl-CoA | - |
Daucus carota | naringenin chalcone + 4 CoA + 3 CO2 | - |
? | |
| caffeoyl-CoA + 3 malonyl-CoA | - |
Daucus carota | eriodictyol + 4 CoA + 3 CO2 | - |
? | |
| malonyl-CoA + 4-coumaroyl-CoA | - |
Daucus carota | naringenin chalcone + CoA + CO2 | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
- |
- |
half-life 3 h in 1.4 mM 2-mercaptoethanol, increase of stability with 14 mM 2-mercaptoethanol | Daucus carota |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
caffeoyl-CoA | Daucus carota |
| 7.9 | - |
4-coumaroyl-CoA | Daucus carota |
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