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Literature summary for 2.3.1.74 extracted from

  • Beerhues, L.; Wiermann, R.
    Chalcone synthases from spinach (Spinacia oleracea L.). I. Purification, peptide patterns, and immunological properties of different forms (1988), Planta, 173, 532-543.
    View publication on PubMed
Search for more literature for 2.3.1.74

General Stability

General Stability Organism
5% activity after freezing for 1 h without presence of glycerol Spinacia oleracea

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0008
-
 4-coumaroyl-CoA isozyme AI Spinacia oleracea
0.001
-
 4-coumaroyl-CoA isozyme AII Spinacia oleracea
0.0019
-
 malonyl-CoA isozyme AII Spinacia oleracea
0.002
-
 malonyl-CoA isozyme AI Spinacia oleracea

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information no activity in chloroplasts Spinacia oleracea
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 isozyme AI, gel filtration, differences in MW may be due to different spatial conformations of AI and AII Spinacia oleracea
62000
-
 isozyme AII, gel filtration, differences in MW may be due to different spatial conformations of AI and AII Spinacia oleracea
78000
-
 isozyme AII, non-denaturing gel electrophoresis, differences in MW may be due to different spatial conformations of AI and AII Spinacia oleracea
88000
-
 isozyme AI, non-denaturing gel electrophoresis, differences in MW may be due to different spatial conformations of AI and AII Spinacia oleracea

Organism

Organism UniProt Comment Textmining
Spinacia oleracea
-
 spinach
-

Purification (Commentary)

Purification (Comment) Organism
-
 Spinacia oleracea

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Spinacia oleracea
-

Storage Stability

Storage Stability Organism
-20°C, 0.4 M phosphate buffer, pH 6.5, 20% v/v glycerol, 4 weeks Spinacia oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
malonyl-CoA + 4-coumaroyl-CoA
-
 Spinacia oleracea naringenin chalcone + CoA + CO2
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 78000 + 1 * 88000, non-denaturating PAGE, 1 * 48000 + 1 * 62000, gel filtration Spinacia oleracea

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 half-life 4-5 d, partially purified enzyme Spinacia oleracea