General Stability | Organism |
---|---|
freezing inactivates | Bos taurus |
high ionic strength stabilizes | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Bos taurus | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47000 | - |
gel filtration | Bos taurus |
50900 | - |
1 * 50900, SDS-PAGE | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
choloyl-CoA + glycine | Bos taurus | conjugation of bile acids in the liver with glycine or taurine prior to being secreted into the bile duct | CoA + glycocholate | - |
? | |
choloyl-CoA + taurine | Bos taurus | conjugation of bile acids in the liver with glycine or taurine prior to being secreted into the bile duct | CoA + taurocholate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
900fold | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
35.2 | - |
taurine | Bos taurus |
46.9 | - |
glycine | Bos taurus |
Storage Stability | Organism |
---|---|
0°C, 50 mM phosphate buffer, pH 7.8, with 0.5 M NaCl, 2 mM dithioerythritol, 1 month, 25% loss of activity | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
choloyl-CoA + glycine | - |
Bos taurus | CoA + glycocholate | - |
? | |
choloyl-CoA + glycine | conjugation of bile acids in the liver with glycine or taurine prior to being secreted into the bile duct | Bos taurus | CoA + glycocholate | - |
? | |
choloyl-CoA + taurine | - |
Bos taurus | CoA + taurocholate | - |
? | |
choloyl-CoA + taurine | conjugation of bile acids in the liver with glycine or taurine prior to being secreted into the bile duct | Bos taurus | CoA + taurocholate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 50900, SDS-PAGE | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
taurocholate stabilizes against temperature denaturation | Bos taurus |
50 | - |
10 min, stable up to | Bos taurus |
57 | - |
10 min, about 70% loss of activity | Bos taurus |
60 | - |
inactivation within 10 min | Bos taurus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | higher kcat for glycine than for taurine | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pI: 6.6 | Bos taurus |