Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli strains DH5alpha and JM105 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G286E | small reduction in activity | Homo sapiens |
I114T | strong reduction of the acetylation activity | Homo sapiens |
K268R | slightly increased activity | Homo sapiens |
additional information | nucleic acid substitutions C282T and C481T have no effect on activity | Homo sapiens |
R197Q | moderate reduction of acetylation activity | Homo sapiens |
R64Q | moderate reduction of acetylation activity | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Homo sapiens | 5829 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
colon | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
N-, O-, and N,O-acetyltransferase activity, wild-type and mutants | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N-hydroxy-2-acetylaminofluorene + N-hydroxy-4-aminobiphenyl | - |
Homo sapiens | N-hydroxy-2-aminofluorene + N-acetoxy-4-aminobiphenyl | - |
r | |
N-hydroxy-4-acetylaminobiphenyl + N-hydroxy-4-aminobiphenyl | - |
Homo sapiens | N-hydroxy-4-aminobiphenyl + N-acetoxy-4-aminobiphenyl | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |