Cloned (Comment) | Organism |
---|---|
gene neuD, DNA and amino acid sequence determination and analysis, phylogenetic analysis, homology modeling of NeuD | Streptococcus sp. |
gene neuD, DNA and amino acid sequence determination and analysis, phylogenetic analysis, homology modeling of NeuD, functional complementation of an enzyme-deficient neuD-mutant Streptococcus sp. strain | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
F88C | single nucleotide polymorphism, associated with variant group B streptococcal O-acetylation phenotype, overview | Streptococcus sp. |
K123A | site-directed mutagenesis, complementation of the COH1DELTANeuD mutant with the K123A mutant of NeuD restores sialylation but not O-acetylation | Streptococcus sp. |
additional information | construction of an enzyme-deficient mutant strain DELTANeuD by allelic replacement mutagenesis, the mutant is functionally complemented by the enzyme from Escherichia coli K1, encoded by gene neuD, the mutant is also analyzed for complementation by the streptococcal K123A mutant, the NeuD-K123A plasmid restores sialic acid biosynthesis to the DELTANeuD mutant, but not the O-acetylation of Neu5Ac | Streptococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + N-acetylneuraminic acid | Streptococcus sp. | - |
CoA + N-acetyl-9-O-acetyl-neuraminate | - |
? | |
acetyl-CoA + N-acetylneuraminic acid | Escherichia coli | - |
CoA + N-acetyl-9-O-acetyl-neuraminate | - |
? | |
additional information | Escherichia coli | Escherichia coli K1, a leading cause of neonatal sepsis and meningitis, expresses a surface CPS containing Neu5Ac, further modified this Sia by O-acetylation | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | Q46674 | strain ATCC 23511, gene neuD | - |
Streptococcus sp. | - |
group B, serotype III GBS strain COH1, gene neuD | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetyl-CoA + N-acetylneuraminic acid | - |
Streptococcus sp. | CoA + N-acetyl-9-O-acetyl-neuraminate | - |
? | |
acetyl-CoA + N-acetylneuraminic acid | - |
Escherichia coli | CoA + N-acetyl-9-O-acetyl-neuraminate | - |
? | |
additional information | Escherichia coli K1, a leading cause of neonatal sepsis and meningitis, expresses a surface CPS containing Neu5Ac, further modified this Sia by O-acetylation | Escherichia coli | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
group B streptococcal sialic acid O-acetyltransferase | - |
Streptococcus sp. |
group B streptococcal sialic acid O-acetyltransferase | - |
Escherichia coli |
NeuD | - |
Streptococcus sp. |
NeuD | - |
Escherichia coli |
Sia O-acetyltransferase | - |
Streptococcus sp. |
Sia O-acetyltransferase | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
acetyl-CoA | - |
Streptococcus sp. | |
acetyl-CoA | - |
Escherichia coli |