BRENDA - Enzyme Database
show all sequences of 2.3.1.4

A missense mutation in the glucosamine-6-phosphate N-acetyltransferase-encoding gene causes temperature-dependent growth defects and ectopic lignin deposition in Arabidopsis

Nozaki, M.; Sugiyama, M.; Duan, J.; Uematsu, H.; Genda, T.; Sato, Y.; Plant Cell 24, 3366-3379 (2012)

Data extracted from this reference:

Engineering
Amino acid exchange
Commentary
Organism
G68S
the mutant called lignescens, a temperature-sensitive mutant that exhibits ectopic lignin deposition and growth defects under high-temperature conditions, is due to single base transition G68S in glucosamine-6-phosphate N-acetyltransferase. When exposed to the restrictive temperature, the mutant strain contains a significantly smaller amount of UDP-GlcNAc than the wild type. The growth defects and ectopic lignification of the mutant are suppressed by the addition of UDP-GlcNAc. N-glycans are reduced and luminal binding protein 3, a typical UPR gene, is expressed in the mutant strain at the restrictive temperature. Treatment with UPR-inducing reagents phenocopies the mutant
Arabidopsis thaliana
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabidopsis thaliana
-
-
-
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
G68S
the mutant called lignescens, a temperature-sensitive mutant that exhibits ectopic lignin deposition and growth defects under high-temperature conditions, is due to single base transition G68S in glucosamine-6-phosphate N-acetyltransferase. When exposed to the restrictive temperature, the mutant strain contains a significantly smaller amount of UDP-GlcNAc than the wild type. The growth defects and ectopic lignification of the mutant are suppressed by the addition of UDP-GlcNAc. N-glycans are reduced and luminal binding protein 3, a typical UPR gene, is expressed in the mutant strain at the restrictive temperature. Treatment with UPR-inducing reagents phenocopies the mutant
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
malfunction
the mutant called lignescens, a temperature-sensitive mutant that exhibits ectopic lignin deposition and growth defects under high-temperature conditions, is due to single base transition G68S in glucosamine-6-phosphate N-acetyltransferase. When exposed to the restrictive temperature, the mutant strain contains a significantly smaller amount of UDP-GlcNAc than the wild type. The growth defects and ectopic lignification of the mutant are suppressed by the addition of UDP-GlcNAc. N-glycans are reduced and luminal binding protein 3, a typical UPR gene, is expressed in the mutant strain at the restrictive temperature. Treatment with UPR-inducing reagents phenocopies the mutant
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
malfunction
the mutant called lignescens, a temperature-sensitive mutant that exhibits ectopic lignin deposition and growth defects under high-temperature conditions, is due to single base transition G68S in glucosamine-6-phosphate N-acetyltransferase. When exposed to the restrictive temperature, the mutant strain contains a significantly smaller amount of UDP-GlcNAc than the wild type. The growth defects and ectopic lignification of the mutant are suppressed by the addition of UDP-GlcNAc. N-glycans are reduced and luminal binding protein 3, a typical UPR gene, is expressed in the mutant strain at the restrictive temperature. Treatment with UPR-inducing reagents phenocopies the mutant
Arabidopsis thaliana
Other publictions for EC 2.3.1.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736197
Piacente
Characterization of a UDP-N-ac ...
Mimivirus
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2014
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Liu
Spatial modulation of key path ...
Bacillus subtilis, Bacillus subtilis BSGN4
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Riegler
Crystal structure and function ...
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Biochem. J.
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2012
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Rodriguez-Diaz
Regulatory insights into the p ...
Lactobacillus casei
Bioengineered
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2012
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Rodriguez-Diaz
Metabolic engineering of Lacto ...
Lactobacillus casei
Biotechnol. Bioeng.
109
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2012
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720693
Nozaki
A missense mutation in the glu ...
Arabidopsis thaliana
Plant Cell
24
3366-3379
2012
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Design and synthesis of novel ...
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Expression, essentiality, and ...
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Li
An enzyme-coupled assay for am ...
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Purification, crystallization ...
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Acta Crystallogr. Sect. F
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Engineering a new pathway for ...
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2006
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Kato
Mosquito glucosamine-6-phospha ...
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Insect Biochem. Mol. Biol.
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2005
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Deng
Metabolic engineering of Esche ...
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2005
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A novel short-root gene encode ...
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Transcription regulation is de ...
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Boehmelt
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Mus musculus
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Vessal
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Partial purification and kinet ...
Homo sapiens
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121B
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1998
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Porowski
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Sus scrofa
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1-12
1990
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Corfield
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Rattus norvegicus
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486904
Giddings
Partial purification and prope ...
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1974
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1
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2
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1
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1
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1
1
1
2
-
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1
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486906
Vessal
Partial purification and prope ...
Vigna radiata var. radiata
Plant Physiol.
51
1055-1060
1973
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-
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-
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4
2
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1
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2
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1
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3
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1
1
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1
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4
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2
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1
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1
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3
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1
1
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1
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486903
Davison
-
Glucoseamine 6-phosphate N-ace ...
Neurospora crassa, Neurospora crassa 5297a
Methods Enzymol.
9
704-707
1966
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-
-
-
-
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1
2
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3
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1
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2
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2
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1
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1
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1
1
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1
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1
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2
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1
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2
-
2
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1
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1
-
1
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486901
Pattabiraman
Purification of glucosamine-6- ...
Neurospora crassa, Ovis aries
Biochim. Biophys. Acta
59
681-689
1962
2
-
-
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5
2
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2
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2
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1
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1
1
1
3
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2
1
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1
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2
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1
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5
-
2
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2
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1
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1
1
1
3
-
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2
1
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-
486902
Davison
Glucosamine metabolism. II. St ...
Canis lupus familiaris, Homo sapiens, Neurospora crassa, Oryctolagus cuniculus, Penicillium sp., Streptococcus sp.
J. Biol. Chem.
226
125-133
1957
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2
2
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6
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1
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7
1
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6
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1
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1
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1
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2
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2
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1
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7
1
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6
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1
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