Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutants in Escherichia coli | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
G142C | glycine-rich motif mutant, 15fold increased dissociation constant value for binding of cofactor pyridoxal 5'-phosphate, 6% turnover compared to the wild-type, 4fold increase of Km-value for glycine | Mus musculus |
G144A | glycine-rich motif mutant, 8.5fold increased dissociation constant value for binding of cofactor pyridoxal 5'-phosphate, 43% turnover compared to the wild-type, unaltered Km-values for the substrates | Mus musculus |
G144S | glycine-rich motif mutant, 8fold increased dissociation constant value for binding of cofactor pyridoxal 5'-phosphate, 39% turnover compared to the wild-type, unaltered Km-values for the substrates | Mus musculus |
G144T | glycine-rich motif mutant, 24.5fold increased dissociation constant value for binding of cofactor pyridoxal 5'-phosphate, 21% turnover compared to the wild-type, unaltered Km-values for the substrates | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00064 | - |
succinyl-CoA | recombinant mutant G144A | Mus musculus | |
0.0012 | - |
succinyl-CoA | recombinant erythroid isoform and mutant G144S | Mus musculus | |
0.0019 | - |
succinyl-CoA | recombinant mutant G144T | Mus musculus | |
0.002 | - |
succinyl-CoA | recombinant mutant G142C | Mus musculus | |
0.002 | - |
succinyl-CoA | recombinant mutant G142C | Rattus norvegicus | |
6.2 | - |
glycine | recombinant mutant G144T | Mus musculus | |
9.3 | - |
glycine | recombinant mutant G144A | Mus musculus | |
11.9 | - |
glycine | recombinant mutant G144S | Mus musculus | |
12.5 | - |
glycine | recombinant erythroid isoform | Mus musculus | |
52.2 | - |
glycine | recombinant mutant G142C | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutants from E. coli | Mus musculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 | Tyr121, Asp279, Arg439, and Lys313 are involved in substrate and cofactor binding, mechanism, subunit localisation | Mus musculus | |
succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 | pyridoxal 5'-phosphate binding site, sequence and function of glycine-rich motif | Mus musculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinyl-CoA + glycine | - |
Mus musculus | 5-aminolevulinate + CoA + CO2 | - |
? | |
succinyl-CoA + glycine | - |
Rattus norvegicus | 5-aminolevulinate + CoA + CO2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
additional information | - |
thermal stability of glycine-rich motif mutants is reduced compared to the wild-type | Mus musculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0183 | - |
succinyl-CoA | recombinant erythroid isoform mutant G142C | Mus musculus | |
0.0633 | - |
succinyl-CoA | recombinant erythroid isoform mutant G144T | Mus musculus | |
0.12 | - |
succinyl-CoA | recombinant erythroid isoform mutant G144S | Mus musculus | |
0.132 | - |
succinyl-CoA | recombinant erythroid isoform mutant G144A | Mus musculus | |
0.308 | - |
succinyl-CoA | recombinant erythroid isoform | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Mus musculus | |
pyridoxal 5'-phosphate | - |
Rattus norvegicus |