Activating Compound | Comment | Organism | Structure |
---|---|---|---|
L-arginine | - |
Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
E280A | change of resiude to the corresponding Escherichia coli residue. Mutation abolishes arginine inhibition and decreases synthase activity | Xanthomonas campestris |
E354A | change of residue to the corresponding Escherichia coli residue. Mutation abolishes arginine activation | Mus musculus |
F121C | change of residue to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation abolishes arginine activation | Mus musculus |
F35C | change of resiude to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation leads to partial inhibition of both synthase and kinase activities by arginine and decrease in synthase activity | Xanthomonas campestris |
G286P | change of resiude to the corresponding Escherichia coli residue. Mutation abolishes arginine inhibition and decreases synthase activity | Xanthomonas campestris |
G288S | change of resiude to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation abolishes arginine inhibition and decreases synthase activity | Xanthomonas campestris |
G360P | change of residue to the corresponding Escherichia coli residue. Mutation abolishes arginine activation | Mus musculus |
G362S | change of residue to the corresponding residue in an arginine-resistant Escherichia coli mutant. Mutation abolishes arginine activation | Mus musculus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
arginine | - |
Xanthomonas campestris |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Xanthomonas campestris | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.2 | - |
mutant G362S | Mus musculus |
3.2 | - |
mutant G362S, presence of 1 mM L-arginine | Mus musculus |
3.4 | - |
mutant G286P, presence of 1 mM L-arginine | Xanthomonas campestris |
3.5 | - |
mutant G286P | Xanthomonas campestris |
4.3 | - |
mutant F35C, presence of 1 mM L-arginine | Xanthomonas campestris |
9.7 | - |
mutant F35C | Xanthomonas campestris |
12.2 | - |
mutant F121C | Mus musculus |
12.3 | - |
wild-type | Mus musculus |
12.3 | - |
wild-type, presence of 1 mM L-arginine | Mus musculus |
12.6 | - |
mutant G360P, presence of 1 mM L-arginine | Mus musculus |
13 | - |
mutant G360P | Mus musculus |
13.1 | - |
mutant E354A, presence of 1 mM L-arginine | Mus musculus |
15.3 | - |
mutant F121C, presence of 1 mM L-arginine | Mus musculus |
15.5 | - |
mutant E354A | Mus musculus |
26.7 | - |
mutant E280A, presence of 1 mM L-arginine | Xanthomonas campestris |
26.9 | - |
mutant E280A | Xanthomonas campestris |
42.6 | - |
mutant G288S, presence of 1 mM L-arginine | Xanthomonas campestris |
46 | - |
mutant G288S | Xanthomonas campestris |
71 | - |
wild-type | Xanthomonas campestris |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N2-acetyl-L-ornithine + L-glutamate | - |
Mus musculus | L-ornithine + N-acetyl-L-glutamate | - |
? | |
N2-acetyl-L-ornithine + L-glutamate | - |
Xanthomonas campestris | L-ornithine + N-acetyl-L-glutamate | - |
? |