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Literature summary for 2.3.1.30 extracted from
- Purification and characterization of L-serine transacetylase and O-acetyl-L-serine sulfhydrylase from kidney bean seedlings (Phaseolus vulgaris) (1971), Biochim. Biophys. Acta, 227, 288-295.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CoA | - |
Phaseolus vulgaris |  |
| D-cysteine | - |
Phaseolus vulgaris | |
| hydroxylamine | - |
Phaseolus vulgaris | |
| L-cysteine | - |
Phaseolus vulgaris | |
| L-homocysteine | weak | Phaseolus vulgaris | |
| L-methionine | weak | Phaseolus vulgaris | |
| p-chloromercuribenzoate | - |
Phaseolus vulgaris | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.2 | - |
acetyl-CoA | - |
Phaseolus vulgaris | |
| 0.6 | - |
L-serine | - |
Phaseolus vulgaris | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phaseolus vulgaris | - |
kidney bean | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partially | Phaseolus vulgaris |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seedling | - |
Phaseolus vulgaris | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.89 | - |
- |
Phaseolus vulgaris |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, 1 week, stable | Phaseolus vulgaris |
| 2°C, 50% loss of activity after 1 week | Phaseolus vulgaris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acetyl-CoA + L-serine | specific for L-serine | Phaseolus vulgaris | CoA + O-acetyl-L-serine | - |
? | |
| acetyl-CoA + L-threonine | no activity | Phaseolus vulgaris | CoA + O-acetyl-L-threonine | - |
? | |
| additional information | no activity: O-acetylserine, O-acetylhomoserine | Phaseolus vulgaris | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Phaseolus vulgaris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
half-life: 25 min | Phaseolus vulgaris |
| 60 | - |
half-life: 2 min | Phaseolus vulgaris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.3 | 8.5 | - |
Phaseolus vulgaris |
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