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Literature summary for 2.3.1.297 extracted from
- LASS3 (longevity assurance homologue 3) is a mainly testis-specific (dihydro)ceramide synthase with relatively broad substrate specificity (2006), Biochem. J., 398, 531-538 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene CERS3, DNA and amino acid sequence determination and analysis, cloning of two transcriptional variants of LASS3 cDNA, which encode a 384-amino-acid protein (LASS3) and a 419-amino-acid protein (LASS3-long), reverse transcription (RT)-PCR expression analysis, recombinant expression in HEK-293T cells | Mus musculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| fumonisin B1 | - |
Mus musculus |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | LASS3 has up to six transmembrane spanning domains | Mus musculus | 16020 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| sphinganine + arachidoyl-CoA | Mus musculus | - |
N-arachidoylsphinganine + CoA | - |
? | |
| sphinganine + behenoyl-CoA | Mus musculus | - |
N-behenoylsphinganine + CoA | - |
? | |
| sphinganine + cerotoyl-CoA | Mus musculus | - |
N-cerotoylsphinganine + CoA | - |
? | |
| sphinganine + lignoceroyl-CoA | Mus musculus | - |
N-lignoceroylsphinganine + CoA | - |
? | |
| sphinganine + palmitoyl-CoA | Mus musculus | - |
N-palmitoylsphinganine + CoA | - |
? | |
| sphinganine + stearoyl-CoA | Mus musculus | - |
N-stearoylsphinganine + CoA | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | Q1A3B0 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no glycoprotein | LASS3 has no putative N-glycosylation site | Mus musculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| testis | Lass3 is mainly testis-specific | Mus musculus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | enzyme LASS3 shows a relatively broad substrate specificity. Both LASS3 isoforms increase the production of several ceramide species, including C18:0- and C24:0-ceramides. LASS3 exhibits relatively low substrate specificity toward fatty acyl-CoAs. Lysates prepared from LASS3-overproducing cells show significant activity toward several fatty acyl-CoAs, including C18:0-, C22:0-, and C24:0-CoA, with the highest activity for C18:0. C16:0-, C20:0-, and C26:0-CoA are also used as substrates by LASS3, albeit with low activity. LASS3-overproducing cells synthesize ceramide from C18:0-CoA with the highest activity at all concentrations tested. This in vitro result is quite consistent with the in vivo results | Mus musculus | ? | - |
? | |
| sphinganine + arachidoyl-CoA | - |
Mus musculus | N-arachidoylsphinganine + CoA | - |
? | |
| sphinganine + arachidoyl-CoA | low activity | Mus musculus | N-arachidoylsphinganine + CoA | - |
? | |
| sphinganine + behenoyl-CoA | - |
Mus musculus | N-behenoylsphinganine + CoA | - |
? | |
| sphinganine + behenoyl-CoA | moderate activity | Mus musculus | N-behenoylsphinganine + CoA | - |
? | |
| sphinganine + cerotoyl-CoA | - |
Mus musculus | N-cerotoylsphinganine + CoA | - |
? | |
| sphinganine + cerotoyl-CoA | low activity | Mus musculus | N-cerotoylsphinganine + CoA | - |
? | |
| sphinganine + lignoceroyl-CoA | - |
Mus musculus | N-lignoceroylsphinganine + CoA | - |
? | |
| sphinganine + lignoceroyl-CoA | high activity | Mus musculus | N-lignoceroylsphinganine + CoA | - |
? | |
| sphinganine + palmitoyl-CoA | - |
Mus musculus | N-palmitoylsphinganine + CoA | - |
? | |
| sphinganine + palmitoyl-CoA | low activity | Mus musculus | N-palmitoylsphinganine + CoA | - |
? | |
| sphinganine + stearoyl-CoA | - |
Mus musculus | N-stearoylsphinganine + CoA | - |
? | |
| sphinganine + stearoyl-CoA | preferred substrate | Mus musculus | N-stearoylsphinganine + CoA | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 46100, about, isozyme LASS3, sequence calculation, x * 50000, about, isozyme LASS3-long, sequence calculation | Mus musculus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| (dihydro)ceramide synthase | - |
Mus musculus |
| ceramide synthase 3 | - |
Mus musculus |
| CerS3 | - |
Mus musculus |
| LASS3 | - |
Mus musculus |
| longevity assurance homologue 3 | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Mus musculus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| additional information | two transcriptional variants of mouse LASS3 cDNA encoding the LASS3 isoforms, which differ in transcriptional initiation sites and polypeptides length, LASS3 and LASS3-long | Mus musculus |
| physiological function | overproduction of LASS3 causes increased levels of C18:0-, C22:0-, and C24:0-ceramides | Mus musculus |
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