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Literature summary for 2.3.1.294 extracted from
- Purification and biochemical characterization of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthases KasA and KasB (2001), J. Biol. Chem., 276, 47029-47037 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21(DE3) cells | Mycobacterium tuberculosis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cerulenin | 37-41% inhibition at 0.1 mM | Mycobacterium tuberculosis |  |
| thiolactomycin | 64-65% inhibition at 0.1 mM | Mycobacterium tuberculosis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0014 | - |
palmitoyl-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 0.0019 | - |
C20:0-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 0.0135 | - |
malonyl-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | Mycobacterium tuberculosis | - |
an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mycobacterium tuberculosis | - |
- |
- |
| Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Q-Sepharose column chromatography and HiTrap column chromatography | Mycobacterium tuberculosis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.0034 | - |
with palmitoyl-CoA as substrate, at pH 7.0 and 37°C | Mycobacterium tuberculosis |
| 0.0318 | - |
palmitoyl-[acyl-carrier protein] as substrate, at pH 7.0 and 37°C | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| an ultra-long-chain di-unsaturated acyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] | - |
Mycobacterium tuberculosis | an ultra-long-chain di-unsaturated 3-oxo-fatty acyl-[acyl-carrier protein] + CO2 + an [acyl-carrier protein] | - |
? | |
| C12:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | very low activity | Mycobacterium tuberculosis | ? | - |
? | |
| C20:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | very low activity | Mycobacterium tuberculosis | ? | - |
? | |
| C4:0-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | very low activity | Mycobacterium tuberculosis | ? | - |
? | |
| palmitoyl-CoA + malonyl-[acyl-carrier protein from Escherichia coli] | weak activity | Mycobacterium tuberculosis | ? | - |
? | |
| palmitoyl-[acyl-carrier protein from Escherichia coli] + malonyl-[acyl-carrier protein from Escherichia coli] | best substrate | Mycobacterium tuberculosis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 45000, SDS-PAGE | Mycobacterium tuberculosis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-ketoacyl-ACP synthase | - |
Mycobacterium tuberculosis |
| beta-ketoacyl-acyl carrier protein synthase | - |
Mycobacterium tuberculosis |
| KasB | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
- |
Mycobacterium tuberculosis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 42 | the enzyme displays about 2-3fold higher specific activities at 37°C compared with 30 or 42°C | Mycobacterium tuberculosis |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
C20:0-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 0.023 | - |
malonyl-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 0.027 | - |
palmitoyl-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | 0.05 mM NADPH is used in assay conditions | Mycobacterium tuberculosis | |
IC50 Value
| IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|
| 0.09 | - |
at pH 7.0 and 37°C | Mycobacterium tuberculosis | thiolactomycin | |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.67 | - |
malonyl-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 5.33 | - |
C20:0-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis | |
| 18.33 | - |
palmitoyl-[acyl-carrier protein from Escherichia coli] | at pH 7.0 and 37°C | Mycobacterium tuberculosis |
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