Literature summary for extracted from

  • Choi, Y.; Johannes, T.; Simurdiak, M.; Shao, Z.; Lu, H.; Zhao, H.
    Cloning and heterologous expression of the spectinabilin biosynthetic gene cluster from Streptomyces spectabilis (2010), Mol. Biosyst., 6, 336-338 .
    View publication on PubMed


Organism UniProt Comment Textmining
Streptomyces spectabilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrobenzoyl-CoA + malonyl-CoA + 6 (S)-methylmalonyl-CoA + 6 NADPH + 4 H+
Streptomyces spectabilis demethyldeoxyspectinabilin + 7 CO2 + 8 CoA + 6 NADP+ + 5 H2O

General Information

General Information Comment Organism
physiological function the polyketidsynthase system consists of 4 subunits. SpnA is an iterative modular type I polyketide synthase. Six PKS modules of the spectinabilin cluster catalyze seven rounds of elongation and reduction. SpnA' is a biomodular protein of the same domain architecture of SpnA (ketosynthase, acyl transferase, dehydratase, ketoreductase and acyl carrier protein), which is responsible for two successive unsaturated chain elongation steps using methylmalonyl-CoA. SpnB contains a sequence of malonyl-CoA specificity in the AT domain, which leads to a full reduction of the beta-keto group after condensation of malonyl-coA.7 SpnC carries two modules of ketosynthase, acyl transferase, and acyl carrier protein domains and a C-terminal thioesterase domain and catalyzes the last two Claisen condensations Streptomyces spectabilis