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Literature summary for 2.3.1.286 extracted from

  • Teng, Y.B.; Jing, H.; Aramsangtienchai, P.; He, B.; Khan, S.; Hu, J.; Lin, H.; Hao, Q.
    Efficient demyristoylase activity of SIRT2 revealed by kinetic and structural studies (2015), Sci. Rep., 5, 8529.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.286

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with BHJH-TM1 protein, hanging drop vapor diffusion method, using 25% (w/v) PEG 3350, 0.1 M HEPES buffer, pH 7.5 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00024
-
 [histone H3]-N6-myristoyl-L-lysine9 at pH 8.0 and 37°C Homo sapiens
0.019
-
 [histone H3]-N6-acetyl-L-lysine9 at pH 8.0 and 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
NAD+ + [protein]-N6-acetyl-L-lysine Homo sapiens
-
 nicotinamide + [protein]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
NAD+ + [protein]-N6-myristoyl-L-lysine Homo sapiens demyristoylase activity of SIRT2 is 5fold more efficient than its deacetylase activity nicotinamide + [protein]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q8IXJ6
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex G200 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
NAD+ + [BHJH-TM1 protein]-N6-myristoyl-L-lysine
-
 Homo sapiens nicotinamide + [BHJH-TM1 protein]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
NAD+ + [histone H2B]-N6-acetyl-L-lysine12
-
 Homo sapiens nicotinamide + [histone H2B]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
NAD+ + [histone H2B]-N6-myristoyl-L-lysine12
-
 Homo sapiens nicotinamide + [histone H2B]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
NAD+ + [histone H3]-N6-acetyl-L-lysine9
-
 Homo sapiens nicotinamide + [histone H3]-L-lysine9 + 2'-O-acetyl-ADP-ribose
-
 ?
NAD+ + [histone H3]-N6-myristoyl-L-lysine9
-
 Homo sapiens nicotinamide + [histone H3]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
NAD+ + [histone H4]-N6-acetyl-L-lysine16
-
 Homo sapiens nicotinamide + [histone H4]-L-lysine16 + 2'-O-acetyl-ADP-ribose
-
 ?
NAD+ + [histone H4]-N6-myristoyl-L-lysine16
-
 Homo sapiens nicotinamide + [histone H4]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?
NAD+ + [protein]-N6-acetyl-L-lysine
-
 Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-acetyl-ADP-ribose
-
 ?
NAD+ + [protein]-N6-myristoyl-L-lysine demyristoylase activity of SIRT2 is 5fold more efficient than its deacetylase activity Homo sapiens nicotinamide + [protein]-L-lysine + 2'-O-myristoyl-ADP-ribose
-
 ?

Synonyms

Synonyms Comment Organism
SIRT2
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.018
-
 [histone H3]-N6-myristoyl-L-lysine9 at pH 8.0 and 37°C Homo sapiens
0.275
-
 [histone H3]-N6-acetyl-L-lysine9 at pH 8.0 and 37°C Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+ dependent on Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
14.5
-
 [histone H3]-N6-acetyl-L-lysine9 at pH 8.0 and 37°C Homo sapiens
74
-
 [histone H3]-N6-myristoyl-L-lysine9 at pH 8.0 and 37°C Homo sapiens