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Literature summary for 2.3.1.28 extracted from

  • Fitton, J.E.; Shaw, W.V.
    Comparison of chloramphenicol acetyltransferase variants in staphylococci. Purification, inhibitor studies and N-terminal sequences (1979), Biochem. J., 177, 575-582.
    View publication on PubMedView publication on EuropePMC
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KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00247
-
 chloramphenicol enzyme variant C Staphylococcus aureus
0.00258
-
 chloramphenicol
-
 Staphylococcus sp.
0.0027
-
 chloramphenicol enzyme variant D Staphylococcus aureus
0.00272
-
 chloramphenicol
-
 Staphylococcus epidermidis
0.0465
-
 acetyl-CoA enzyme variant D Staphylococcus aureus
0.0561
-
 acetyl-CoA
-
 Staphylococcus epidermidis
0.0573
-
 acetyl-CoA
-
 Staphylococcus sp.
0.0609
-
 acetyl-CoA enzyme variant C Staphylococcus aureus

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
 enzyme variant C and D
-
Staphylococcus epidermidis
-
 enzyme variant B
-
Staphylococcus sp.
-
 enzyme type A
-

Oxidation Stability

Oxidation Stability Organism
photooxidation of enzyme type C in presence of methylene blue, progressive loss of activity with increase in pH, inclusion of 1 mM chloramphenicol affords complete protection against loss of activity Staphylococcus aureus

Purification (Commentary)

Purification (Comment) Organism
enzyme variant A Staphylococcus sp.
enzyme variant B Staphylococcus epidermidis
enzyme variant C and D Staphylococcus aureus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Staphylococcus aureus
additional information
-
-
 Staphylococcus epidermidis
additional information
-
-
 Staphylococcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + chloramphenicol
-
 Staphylococcus aureus CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol
-
 Staphylococcus epidermidis CoA + chloramphenicol 3-acetate
-
 ?
acetyl-CoA + chloramphenicol
-
 Staphylococcus sp. CoA + chloramphenicol 3-acetate
-
 ?