Crystallization (Comment) | Organism |
---|---|
using 100mM Tris-HCl pH 7.0, 230 mM MgCl2 and 12-16% (w/v) PEG 8000 | Mycolicibacterium smegmatis |
Protein Variants | Comment | Organism |
---|---|---|
D131A | the mutant shows about 35% activity compared to the wild type enzyme | Mycolicibacterium smegmatis |
E149A | the mutant shows about 75% activity compared to the wild type enzyme | Mycolicibacterium smegmatis |
E200A | the mutant shows less than 3% activity compared to the wild type enzyme | Mycolicibacterium smegmatis |
F182W/L197W | the mutant shows less than 3% activity compared to the wild type enzyme | Mycolicibacterium smegmatis |
H126A | the mutant shows less than 3% activity compared to the wild type enzyme | Mycolicibacterium smegmatis |
H284A | the mutant shows about 50% activity compared to the wild type enzyme | Mycolicibacterium smegmatis |
R164A | the mutant shows about 78% activity compared to the wild type enzyme | Mycolicibacterium smegmatis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Mycolicibacterium smegmatis | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycolicibacterium smegmatis | A0QWG5 | - |
- |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | A0QWG5 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography | Mycolicibacterium smegmatis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol | - |
Mycolicibacterium smegmatis | CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol | - |
? | |
palmitoyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol | - |
? | |
palmitoyl-CoA + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol | - |
Mycolicibacterium smegmatis | CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? | |
palmitoyl-CoA + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol | - |
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 | CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PatA | - |
Mycolicibacterium smegmatis |
Rv2611c | - |
Mycolicibacterium smegmatis |