Literature summary for 2.3.1.255 extracted from

Lee, M.N.; Kweon, H.Y.; Oh, G.T.
N-alpha-acetyltransferase 10 (NAA10) in development the role of NAA10 (2018), Exp. Mol. Med., 50, 1-11 .

Search for more literature for 2.3.1.255

Protein Variants

Protein Variants	Comment	Organism
F128I	site-directed mutagenesis, the mutation leads an altered structure and reduced stability, and a dramatic recuction of Nt catalytic activity compared to wild-type	Homo sapiens
F128L	site-directed mutagenesis, the mutation leads an altered structure and reduced stability, and a dramatic recuction of Nt catalytic activity compared to wild-type	Homo sapiens
additional information	enzyme knockdown by siRNA. Generation of mutant daf-31(m655) by removal of 151 bp of promoter upstream of the ATG start codon and 242 bp of daf-31 coding region dowstream of the ATG start codon. Generation of and overexpression mutant daf-31 OE for which the full-length dar-31 genomic DNA is cloned into pGEM-T vector. Generation of mutant vncBDk by with impaired N-terminal activity	Caenorhabditis elegans
additional information	generation of an Ard1 null mutant by removal of the ARD1 coding region	Trypanosoma brucei
additional information	generation of enzyme mutant by frame shift mutation causing a acetyltransferase-truncated enzymatic region, and of another mutant vnc by intron-insertion mutation	Drosophila melanogaster
additional information	generation of mutant naa10-1 and of a naa15 mutant by T-DNA insertion-disrupting gene expression	Arabidopsis thaliana
additional information	generation of mutant naa10MO by morpholino-based knockdown	Danio rerio
additional information	mutation ard1::HIS3 is caused by inserting a Barn HI fragment containinng the HIS3 gene into the Barn HI site of plasmid YCpE18 that lies within the functional sequence of ARD1. Genes nat1/ard1 double mutant nat1-5::LEU;ard1 is generated by mating of nat1 and ard1 single mutants, the single mutants of nat1 (Naa15) and ard1 (Naa10) display identical phenotypes, no additional phenotypes are found in the double mutant. Recombinant expression of the S37P mutant of human Naa10 in a NatA-defective yeast strain, the hNaa10 expressing mutant strain shows a lack of proper complex formation with hNaa15 and reduced in vitro catalytic activity, a decrease of Nt-acetylome and an increase in the Hsp70 family proteins	Saccharomyces cerevisiae
R116W	site-directed mutagenesis, the mutation leads to a reduction in catalytic activity for the peptide substrates EEEI and SESS by 15% compared to wild-type	Homo sapiens
R83C	site-directed mutagenesis, the mutation interferes with acetyl-CoA binding and leads to a 60% reduction in Nt-catalytic activity compared to wild-type	Homo sapiens
S37P	site-directed mutagenesis, the mutant Naa10 protein shows reduced catalytic activity for EEEI, DDDI, and SESS peptide substrates, and inability to combine with Naa15. The mutant hNaa10 S37P recombinantly expressed in a NatA-defective Saccharomyces cerevisiae strain lacks a proper complex formation with hNaa15 and is reduced in in vitro catalytic activity	Homo sapiens
S39P	site-directed mutagenesis, the mutation does not cause a phenotype	Saccharomyces cerevisiae
V107F	site-directed mutagenesis, the mutation leads to a reduction in catalytic activity for the peptide substrates EEEI and SESS by 95% compared to wild-type	Homo sapiens
Y43S	site-directed mutagenesis, the mutant is catalytically impaired in vitro, with approximately an 85% reduction in Nt-catalytic activity for peptide substrates EEEI, DDDI, and SESS	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q9FKI4	-	-
Caenorhabditis elegans	O61219	-	-
Caenorhabditis elegans DAF-31	O61219	-	-
Danio rerio	Q7T3B8	-	-
Drosophila melanogaster	Q9VT75	-	-
Homo sapiens	P41227	-	-
Mus musculus	Q3UX61	-	-
Mus musculus	Q9QY36	-	-
Mus musculus C57Bl6/J	Q3UX61	-	-
Mus musculus C57Bl6/J	Q9QY36	-	-
Saccharomyces cerevisiae	P07347 AND P12945	subunits Naa10 and Naa15 of enzmye complex NatA	-
Saccharomyces cerevisiae ATCC 204508	P07347 AND P12945	subunits Naa10 and Naa15 of enzmye complex NatA	-
Trypanosoma brucei	Q9NFL8	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
bone	-	Mus musculus	-
brain	during brain development in mouse, Naa10 and Naa15 (the auxiliary subunit of NatA) are highly expressed in regions rich in proliferating and migrating cells, such as the ventricular zone, neocortex, olfactory bulb, and hippocampus. The expression of both genes is downregulated as neurons differentiate and mitotic and migratory activities subside. Then, once again, their expression increases during postnatal development in the hippocampus and during the neuronal dendritic development of Purkinje cells (PCs) in the cerebellum. This finding indicates that the regulation of expression of both genes is related to neuronal development, especially in the hippocampus and in the PCs of the cerebellum	Mus musculus	-
cerebellar Purkinje cell	-	Mus musculus	-
colon	-	Homo sapiens	-
colon	-	Mus musculus	-
hippocampus	-	Mus musculus	-
kidney	-	Homo sapiens	-
kidney	-	Mus musculus	-
liver	-	Homo sapiens	-
liver	-	Mus musculus	-
lung	-	Homo sapiens	-
lung	-	Mus musculus	-
additional information	NAA10 and NAA11 display opposite expression patterns during spermatogenesis. No expression of Naa11 in premeiotic spermatogonia	Mus musculus	-
additional information	tissue-specific expression of Naa10 during different developmental stages in C57BL6/J mice	Mus musculus	-
additional information	tissue-specific expression of Naa10 during different developmental stages in humans	Homo sapiens	-
NB-4 cell	NAA10 expression decreases with the induction of differentiation in NB4 cells, but the level of NAA11 remains unchanged	Mus musculus	-
neuron	-	Mus musculus	-
occipital lobe	-	Homo sapiens	-
occipital lobe	-	Mus musculus	-
olfactory bulb	-	Mus musculus	-
osteoblast	-	Mus musculus	-
parietal lobe	-	Homo sapiens	-
parietal lobe	-	Mus musculus	-
skin	-	Homo sapiens	-
skin	-	Mus musculus	-
spermatogonium	Naa10 is expressed in premeiotic spermatogonia	Mus musculus	-
spinal cord	-	Homo sapiens	-
spinal cord	-	Mus musculus	-
testis	NAA11, a homologue of NAA10, is predominantly expressed in mouse testis	Mus musculus	-
testis	NAA11, a homologue of NAA10, is predominantly expressed in mouse testis. NAA10 and NAA11 display opposite expression patterns during spermatogenesis	Mus musculus	-
tongue	-	Homo sapiens	-
tongue	-	Mus musculus	-
uterus	-	Homo sapiens	-
uterus	-	Mus musculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
acetyl-CoA + N-terminal L-aspartyl-[DDIAALRWGRPVGRRRRPVRVYP]	-	Homo sapiens	CoA + H+ + N-terminal Nalpha-acetyl-L-aspartyl-[DDDIAALRWGRPVGRRRRPVRVYP]	-	?
acetyl-CoA + N-terminal L-glutamyl-[EEIAALRWGRPVGRRRRPVRVYP]	-	Homo sapiens	CoA + H+ + N-terminal Nalpha-acetyl-L-glutamyl-[EEEIAALRWGRPVGRRRRPVRVYP]	-	?
acetyl-CoA + N-terminal L-methionyl-[LGPEGGRWGRPVGRRRRPVRVYP]	-	Homo sapiens	CoA + H+ + N-terminal Nalpha-acetyl-L-methionyl-[MLGPEGGRWGRPVGRRRRPVRVYP]	-	?
acetyl-CoA + N-terminal L-seryl-[ESSSKSRWGRPVGRRRRPVRVYP]	-	Homo sapiens	CoA + H+ + N-terminal Nalpha-acetyl-L-seryl-[SESSSKSRWGRPVGRRRRPVRVYP]	-	?
additional information	acetylation of alpha-tubulin	Mus musculus	?	-	-
additional information	enzyme zNaa10 has a predicted N-terminal activity with identical substrate specificity to human Naa10 in vitro	Danio rerio	?	-	-
additional information	acetylation of alpha-tubulin	Mus musculus C57Bl6/J	?	-	-

Synonyms

Synonyms	Comment	Organism
ARD1	-	Danio rerio
ARD1	-	Trypanosoma brucei
ARD1	-	Drosophila melanogaster
ARD1	-	Saccharomyces cerevisiae
Ard1b	-	Mus musculus
ARD2	-	Mus musculus
AtNAA10	-	Arabidopsis thaliana
daf-31	-	Caenorhabditis elegans
N-alpha-acetyltransferase	-	Caenorhabditis elegans
N-alpha-acetyltransferase 10	-	Homo sapiens
N-alpha-acetyltransferase 10	-	Danio rerio
N-alpha-acetyltransferase 10	-	Mus musculus
N-alpha-acetyltransferase 10	-	Arabidopsis thaliana
N-alpha-acetyltransferase 11	-	Mus musculus
NAA10	-	Homo sapiens
NAA10	-	Danio rerio
NAA10	-	Mus musculus
NAA10	-	Arabidopsis thaliana
NAA11	-	Mus musculus
NAT1	-	Saccharomyces cerevisiae
TbARD1	-	Trypanosoma brucei
vnc	-	Caenorhabditis elegans
yNaa10	-	Saccharomyces cerevisiae
yNaa15	-	Saccharomyces cerevisiae
zNaa10	-	Danio rerio

Cofactor

Cofactor	Comment	Organism	Structure
acetyl-CoA	-	Homo sapiens

Expression

Organism	Comment	Expression
Mus musculus	in mouse, the expression of Naa10 is downregulated during meiosis	down
Mus musculus	in mouse, the expression of Naa11 is upregulated during meiosis	up

General Information

General Information	Comment	Organism
malfunction	enzyme knockdown causes a phenotype with lethality, growth retardation, bent axis and tails, abnormal eyes, and less pigmentation	Danio rerio
malfunction	enzyme mutants show phenotypes with pleiotropic oogenesis, aberrant mitosis, egg chamber encapsulation defects, and nurse cell chromatin dispersion defects	Drosophila melanogaster
malfunction	inactive Naa10 mutant S37Pw shows a phenotype with perinatal lethal disorder, hypotonia, global developmental delay, cryptorchidism, cardiac arrhythmias, skin laxity, dysmorphic features, hernias, and large fontanels. Naa10 mutant Y43S shows a phenotype with intellectual disability, facial dysmorphism, scoliosis, and long QT. Mutant R83C shows a phenootype with hypotonia, global developmental delay, dysmorphic features, autism spectrum disorder, epileptic encephalopathy, extrapyramidal signs, hypertension with left ventricular hypertrophy, thin corpus callosum, and progressive white matter loss. Mutations V107F and R116W cause phenotypes with severe global developmental delay with postnatal growth, skeletal anomalies, truncal hypotonia with hypertonia of the extremities, minor facial features, and behavioral anomalies. Mutation of residue F128 causes moderate to severe intellectually disability, feeding difficulties, eye anomalies, hypotonia, and developmental delay	Homo sapiens
malfunction	mutation ard1::HIS3 leads to a defect in transcription of a-specific genes, but permits expression of the information resident at HML. The mutant shows a phenotypes with reduced viability and sensitivity to heat shock and salt, it fails to enter stationay phase, it shows a lack of glycogen accumulation, a sporulation defect, poor mating, and fails to undergo meiosis. The mutant nat1-5::LEU;ard1 is inable to sporulate, has slow growth, reduced mating, inhibited sporulation, and impaired resistance to heat shock. It fails G1 arrest, shows a partial depression of HML, and fails to accumulate storage. yNaa10 deficiency leads to a growth defect, sensitivity to caffeine and cycloheximide, impaired resistance to heat shock, and decreased mating efficiency	Saccharomyces cerevisiae
malfunction	overexpression of gene daf-31 causes an increased lifespan in daf-2 mutant enhancing reproduction, while daf-31 knockdown by siRNA causes a decreased lifespan	Caenorhabditis elegans
malfunction	overexpression of Naa10 in mice results in the delayed closure of calvarial fontanels and reduced bone density, osteoblast surfaces and mRNA levels of the osteoblastogenic genes in calvaria. In contrast, Naa10 deficient mice display calvarial and femoral bone development to a greater extent on postnatal day 3	Mus musculus
malfunction	the ARD1 null mutation leads to impaired growth in bloodstream-form cells and reduced differentiation to insect-stage cells	Trypanosoma brucei
malfunction	the naa10 knockout mutant naa10-1 shows growth retardation in vegetative stage, abortion of embryogenesis, and drought-adapted root morphology, the mutation is lethal. A knockout of naa15 causes the same phenotype	Arabidopsis thaliana
physiological function	enzyme Daf-31 regulates the transcriptional activity of DAF-16, the FOXO transcription factor. Mutant daf-31(m655) leads to developmental larval arrest, fat accumulation, formation of dauer-like larvae under starvation conditions, and decreased lifespan, and the mutant lacks SDS-resistance and cannot resume development and reproduction after food re-providing	Caenorhabditis elegans
physiological function	importance of NAA10 catalytic activity in human development. The potential role of NAA10 varies depending on transcriptional levels in different tissues and embryonic stages during development	Homo sapiens
physiological function	importance of NAA10 catalytic activity in mouse development. The potential role of NAA10 varies depending on transcriptional levels in different tissues and embryonic stages during development. Naa10 homologue Naa11 has a role in the cellular differentiation process while Naa10 has a role in the cellular proliferation process. The differential expression pattern of Naa10 and Naa11 suggests that Naa11 is complementary to Naa10 at least in the mice and that its biological role can be important in spermiogenesis or cellular processes	Mus musculus
physiological function	importance of NAA10 catalytic activity in mouse development. The potential role of NAA10 varies depending on transcriptional levels in different tissues and embryonic stages during development. Naa10 homologue Naa11 has a role in the cellular differentiation process while Naa10 has a role in the cellular proliferation process. The differential expression pattern of Naa10 and Naa11 suggests that Naa11 is complementary to Naa10 at least in the mice and that its biological role can be important in spermiogenesis or cellular processes. Naa10 is known to regulate cellular processes, and its effects are not only catalyzed through its major activity as a NAT but also through the N-epsilon-acetylation of several proteins. The N-epsilon-acetyl-activity of Naa10 requires auto-acetylation. This requirement is similar to that of other acetyltransferases, which acetylate themselves for their catalytic and functional activities. Naa10 plays an important role in osteoblast differentiation and the early phases of bone formation. Naa10 counteracts HDAC6 by acetylating alpha-tubulin, thereby promoting MT stability for dendritic development	Mus musculus
physiological function	Naa10 is crucial for cell growth and sporulation	Saccharomyces cerevisiae