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Literature summary for 2.3.1.24 extracted from

  • Mesicek, J.; Lee, H.; Feldman, T.; Jiang, X.; Skobeleva, A.; Berdyshev, E.V.; Haimovitz-Friedman, A.; Fuks, Z.; Kolesnick, R.
    Ceramide synthases 2, 5, and 6 confer distinct roles in radiation-induced apoptosis in HeLa cells (2010), Cell. Signal., 22, 1300-1307 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.3.1.24

Cloned(Commentary)

Cloned (Comment) Organism
gene CERS5, recombinant FLAG-tagged isozyme overexpression in HeLa cells, quantitative real-time PCR enzyme expression analysis, CerS5 overexpression significantly increases apoptosis in a time- and dose-dependent manner compared to empty vector-transfected controls. CerS2, CerS5, and CerS6 form heterocomplexes in transformed HeLa cells Homo sapiens
gene CERS6, recombinant FLAG-tagged isozyme overexpression in HeLa cells, quantitative real-time PCR enzyme expression analysis, CerS6 overexpression yields no significant differences in IR-induced apoptosis compared to empty vector-transfected cells. CerS2, CerS5, and CerS6 form heterocomplexes in transformed HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information CerS6 knockdown by shRNA Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information nonlinear fitting and Michaelis-Menten kinetic analyses Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrial membrane
-
 Homo sapiens 31966
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sphinganine + palmitoyl-CoA Homo sapiens
-
 N-palmitoylsphinganine + CoA
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6ZMG9
-
-
Homo sapiens Q8N5B7
-
-

Purification (Commentary)

Purification (Comment) Organism
subcellular fractionation of HeLa cells recombinantly expressing the CerS isozyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sphinganine + palmitoyl-CoA
-
 Homo sapiens N-palmitoylsphinganine + CoA
-
 ?

Synonyms

Synonyms Comment Organism
ceramide synthase 5
-
 Homo sapiens
ceramide synthase 6
-
 Homo sapiens
CerS5
-
 Homo sapiens
CerS6
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
 assay at Homo sapiens

Expression

Organism Comment Expression
Homo sapiens ionizing radiation (IR) induces de novo synthesis of ceramide by specifically activating CerS isoforms 2, 5, and 6 up

General Information

General Information Comment Organism
malfunction CerS6 knockdown might decrease apoptosis compared to normal irradiated HeLa cells Homo sapiens
malfunction overexpression of CerS5 increases apoptosis in HeLa cells. CerS2 and CerS5 overexpression significantly alters apoptosis Homo sapiens
metabolism regulation of CerS isozymes, overview. The interplay among the CerS proteins takes place in a stress stimulus-, cell type- and subcellular compartment-specific manner. CerS2 and CerS5 overexpression significantly alters apoptosis, determination of ionizing radiation-induced mitochondrial ceramide elevations via CerS2, 5, and 6, overview. CerS2 and CerS5 overexpressions defines opposing roles in radiation-induced mitochondrial apoptosis Homo sapiens
metabolism regulation of CerS isozymes, overview. The interplay among the CerS proteins takes place in a stress stimulus-, cell type- and subcellular compartment-specific manner. CerS6 overexpression yields no significant differences in IR-induced apoptosis compared to empty vector-transfected cells. Determination of ionizing radiation-induced mitochondrial ceramide elevations via CerS2, 5, and 6, overview Homo sapiens
physiological function selective tissue and subcellular distribution of the six mammalian CerS isoforms, combined with distinct fatty acyl chain length substrate preferences, implicate differential functions of specific ceramide species in cellular signaling. Ionizing radiation (IR) induces de novo synthesis of ceramide to influence HeLa cell apoptosis by specifically activating isozymes CerS isoforms 2, 5, and 6 that generate opposing anti- and pro-apoptotic ceramides in mitochondrial membranes. Isozymes CerS5 and CerS6 each confer about 50% of the C16:0 CerS baseline synthetic activity, both are required for IR-induced activity. IR-induced CerS-mediated ceramide generation, and subsequent apoptosis, occurs in a cell-type specific manner. CerS2 and CerS5 overexpressions defines opposing roles in radiation-induced mitochondrial apoptosis Homo sapiens
physiological function selective tissue and subcellular distribution of the six mammalian CerS isoforms, combined with distinct fatty acyl chain length substrate preferences, implicate differential functions of specific ceramide species in cellular signaling. Ionizing radiation (IR) induces de novo synthesis of ceramide to influence HeLa cell apoptosis by specifically activating isozymes CerS isoforms 2, 5, and 6 that generate opposing anti- and pro-apoptotic ceramides in mitochondrial membranes. Isozymes CerS5 and CerS6 each confer about 50% of the C16:0 CerS baseline synthetic activity, both are required for IR-induced activity. IR-induced CerS-mediated ceramide generation, and subsequent apoptosis, occurs in a cell-type specific manner. CerS6 overexpression yields no significant differences in IR-induced apoptosis compared to empty vector-transfected cells Homo sapiens