Cloned (Comment) | Organism |
---|---|
COS-7 cells are cotransfected with the cDNAs for eNOS and HA-tagged DHHC-21 | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi membrane | isozymes DHHC-2, -3, -7, -8, and -21 | Homo sapiens | 139 | - |
additional information | the isozymes colocalize with eNOS in the Golgi and plasma membrane and interact with eNOS in endothelial cells | Homo sapiens | - |
- |
plasma membrane | isozymes DHHC-2, -3, -7, -8, and -21 | Homo sapiens | 5886 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + [endothelial nitric oxide synthase]-L-cysteine | Homo sapiens | isozyme DHHC-21 | [endothelial nitric oxide synthase]-S-palmitoyl-L-cysteine + CoA | - |
r | |
palmitoyl-CoA + [protein]-L-cysteine | Homo sapiens | - |
[protein]-S-palmitoyl-L-cysteine + CoA | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
endothelial cell | expression of 5 PAT isozymes | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
palmitoyl-CoA + [endothelial nitric oxide synthase]-L-cysteine | isozyme DHHC-21 | Homo sapiens | [endothelial nitric oxide synthase]-S-palmitoyl-L-cysteine + CoA | - |
r | |
palmitoyl-CoA + [protein]-L-cysteine | - |
Homo sapiens | [protein]-S-palmitoyl-L-cysteine + CoA | - |
r |
Synonyms | Comment | Organism |
---|---|---|
Asp-His-His-Cys motif palmitoyl transferase | - |
Homo sapiens |
DHHC palmitoyl transferase | - |
Homo sapiens |
DHHC-2 | - |
Homo sapiens |
DHHC-21 | - |
Homo sapiens |
DHHC-21 palmitoyl transferase | - |
Homo sapiens |
DHHC-3 | - |
Homo sapiens |
DHHC-3 palmitoyl transferase | - |
Homo sapiens |
DHHC-7 | - |
Homo sapiens |
DHHC-8 | - |
Homo sapiens |
Pat | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme is an Asp-His-His-Cys motif (DHHC) palmitoyl transferase family member | Homo sapiens |
malfunction | palmitoylation of wild-type eNOS by DHHC-21 is diminished by mutation of the two sites of eNOS palmitoylation, cysteines 15 and 26, palmitoylation deficient mutants of eNOS, i.e. G2A, C15/26S, and L2S, release less nitric oxide. Inhibition of DHHC-21 palmitoyl transferase, but not DHHC-3, in human endothelial cells reduces eNOS palmitoylation, eNOS targeting, and stimulated NO production | Homo sapiens |
physiological function | regulatory role of DHHC-21 in governing eNOS localization and function. eNOS fatty acylation is required for an efficient interaction with DHHC proteins and NO release, overview | Homo sapiens |