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Literature summary for 2.3.1.224 extracted from

  • Dudareva, N.; D'Auria, J.C.; Nam, K.H.; Raguso, R.A.; Pichersky, E.
    Acetyl-CoA:benzylalcohol acetyltransferase - an enzyme involved in floral scent production in Clarkia breweri (1998), Plant J., 14, 297-304.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Clarkia breweri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48200
-
x * 48200, calculated from sequence Clarkia breweri
58000
-
1 * 58000, SDS-PAGE Clarkia breweri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
acetyl-CoA + benzyl alcohol Clarkia breweri
-
CoA + benzyl acetate
-
?
acetyl-CoA + cinnamyl alcohol Clarkia breweri
-
CoA + cinnamyl acetate
-
?

Organism

Organism UniProt Comment Textmining
Clarkia breweri O64988
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Clarkia breweri

Reaction

Reaction Comment Organism Reaction ID
acetyl-CoA + benzyl alcohol = CoA + benzyl acetate (1) Clarkia breweri
acetyl-CoA + cinnamyl alcohol = CoA + cinnamyl acetate (2) Clarkia breweri

Source Tissue

Source Tissue Comment Organism Textmining
additional information no BEAT mRNA is detected in leaves Clarkia breweri
-
petal petal contain the majority of BEAT transcripts Clarkia breweri
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acetyl-CoA + 2-(naphthalen-2-yl)ethanol at 9% of the activity compared to benzyl alcohol, plant purified enzyme, at 28% of the activity compared to benzyl alcohol (Escherichia coli-expressed enzyme) Clarkia breweri CoA + 2-(naphthalen-2-yl)ethyl acetate
-
?
acetyl-CoA + 2-phenylethanol at 33% of the activity compared to benzyl alcohol, plant purified enzyme, at 57% of the activity compared to benzyl alcohol (Escherichia coli-expressed enzyme) Clarkia breweri CoA + 2-phenylethylacetate
-
?
acetyl-CoA + benzyl alcohol
-
Clarkia breweri CoA + benzyl acetate
-
?
acetyl-CoA + cinnamyl alcohol
-
Clarkia breweri CoA + cinnamyl acetate
-
?
acetyl-CoA + cinnamyl alcohol at 88% of the activity compared to benzyl alcohol (plant purified enzyme), at 97% of the activity compared to benzyl alcohol (Escherichia coli-expressed enzyme) Clarkia breweri CoA + cinnamyl acetate
-
?
additional information no activity with: 2-hydroxybenzylalcohol, 3-hydroxybenzylalcohol, 4-hydroxybenzylalcohol and linalool Clarkia breweri ?
-
?

Subunits

Subunits Comment Organism
? 1 * 58000, SDS-PAGE Clarkia breweri
? x * 48200, calculated from sequence Clarkia breweri

Synonyms

Synonyms Comment Organism
BEAT
-
Clarkia breweri

Expression

Organism Comment Expression
Clarkia breweri three days after anthesis, mRNA levels steeply declines down
Clarkia breweri the levels of BEAT mRNA in the petals increases as the bud matures, and peaks at anthesis up

General Information

General Information Comment Organism
physiological function the enzyme is important for floral scent production Clarkia breweri