Cloned (Comment) | Organism |
---|---|
DNA and amin acid sequence determination and analysis, sequence comparison and phylogenetic tree | Garcinia mangostana |
Protein Variants | Comment | Organism |
---|---|---|
A257G | site-directed mutagenesis, altered substrate entrance in the active site structure compared to the wild-type enzyme. The mutant A257G gives similar enzyme products as the wild type with both benzoyl-CoA and 4-coumaroyl-CoA | Garcinia mangostana |
G339S | site-directed mutagenesis, altered substrate entrance in the active site structure compared to the wild-type enzyme. The mutant G339S yields high amounts of both benzophenone and triketide lactone type with benzoyl-CoA but shows no activity for 4-coumaroyl-CoA | Garcinia mangostana |
G339V | site-directed mutagenesis, altered substrate entrance in the active site structure compared to the wild-type enzyme. The mutant shows no activity with either benzoyl-CoA or 4-coumaroyl-CoA | Garcinia mangostana |
additional information | homology model of wild-type GmBPS and GmBPS mutants A257G, T133L, G339V, and G339S, active-site architectures by surface models and substrate entrances, overview | Garcinia mangostana |
T133L | site-directed mutagenesis, altered substrate entrance in the active site structure compared to the wild-type enzyme. With benzoyl-CoA as a starter, the T133L mutant yields triketide lactone as the major enzymatic product | Garcinia mangostana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00965 | - |
benzoyl-CoA | pH 7.0, 30°C | Garcinia mangostana | |
0.0164 | - |
malonyl-CoA | pH 7.0, 30°C | Garcinia mangostana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42700 | - |
xx * 42700, about, sequence calculation | Garcinia mangostana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + benzoyl-CoA | Garcinia mangostana | - |
4 CoA + 2,4,6-trihydroxybenzophenone + 3 CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Garcinia mangostana | L7NCQ3 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Garcinia mangostana | - |
pericarp | - |
Garcinia mangostana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3 malonyl-CoA + benzoyl-CoA | - |
Garcinia mangostana | 4 CoA + 2,4,6-trihydroxybenzophenone + 3 CO2 | - |
? | |
3 malonyl-CoA + benzoyl-CoA | a minor amount of tetraketide lactone and triketide lactone are also obtained | Garcinia mangostana | 4 CoA + 2,4,6-trihydroxybenzophenone + 3 CO2 | - |
? | |
additional information | the recombinant enzyme produces 2,4,6-trihydroxybenzophenone as the predominant product with benzoyl CoA as substrate. It also accepts other substrates, such as other plant PKSs, and used 1-3 molecules of malonyl CoA to form various phloroglucinol-type and polyketide lactone-type compounds | Garcinia mangostana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | xx * 42700, about, sequence calculation | Garcinia mangostana |
Synonyms | Comment | Organism |
---|---|---|
benzophenone synthase | - |
Garcinia mangostana |
BPS | - |
Garcinia mangostana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Garcinia mangostana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0495 | - |
benzoyl-CoA | pH 7.0, 30°C | Garcinia mangostana | |
0.0582 | - |
malonyl-CoA | pH 7.0, 30°C | Garcinia mangostana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Garcinia mangostana |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Garcinia mangostana | sequence calculation | - |
5.97 |
General Information | Comment | Organism |
---|---|---|
evolution | phylogenetic tree | Garcinia mangostana |
metabolism | the enzyme catalyzes the formation of an intermediate, 2,4,6-trihydroxybenzophenone, in the biosynthetic pathway of alpha-mangostin | Garcinia mangostana |
additional information | the catalytic triad is formed by Cys164, His303, and Asn336 | Garcinia mangostana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.55 | - |
malonyl-CoA | pH 7.0, 30°C | Garcinia mangostana | |
5.14 | - |
benzoyl-CoA | pH 7.0, 30°C | Garcinia mangostana |