BRENDA - Enzyme Database
show all sequences of 2.3.1.210

Crystal structure of TDP-fucosamine acetyltransferase (WecD) from Escherichia coli, an enzyme required for enterobacterial common antigen synthesis

Hung, M.N.; Rangarajan, E.; Munger, C.; Nadeau, G.; Sulea, T.; Matte, A.; J. Bacteriol. 188, 5606-5617 (2006) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene wecD, expression of N-terminal fusion protein with a noncleavable His6-tag in Escherichia coli strain BL21(DE3)
Escherichia coli
Crystallization (Commentary)
Crystallization (Commentary)
Organism
purified recombinant N-terminally His6-tagged WecD free and in complex with acetyl-CoA, hanging drop vapour diffusion method, mixing of 0.001 ml of 7.1 mg/ml protein in a 20 mM Tris-Cl, pH 8.0, 450 mM NaCl, and 5 mM DTT, with 0.001 ml reservoir solution aontaining 100 mM Na-acetate, pH 4.5, 10 mM ZnSO4, 20°C, 1 day, X-ray diffraction structure determination and analysis at 1.66-1.95 A resolution
Escherichia coli
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
Escherichia coli
-
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
Escherichia coli CFT073
-
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
Q8FBQ3
gene wecD
-
Escherichia coli CFT073
Q8FBQ3
gene wecD
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant N-terminally His6-tagged WecD from Escherichia coli strain BL21(DE3)
Escherichia coli
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
-
719706
Escherichia coli
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
-
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
acetyl-CoA binding site structure, overview
719706
Escherichia coli
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
-
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
-
719706
Escherichia coli CFT073
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
-
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
acetyl-CoA binding site structure, overview
719706
Escherichia coli CFT073
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
WecD is a dimeric enzyme, with each monomer adopting the GNAT N-acetyltransferase fold, gel filtration and dynamic light scattering, three-dimensional structure comparison, overview
Escherichia coli
Synonyms
Synonyms
Commentary
Organism
TDP-fucosamine acetyltransferase
-
Escherichia coli
WecD
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
acetyl-CoA
-
Escherichia coli
Cloned(Commentary) (protein specific)
Commentary
Organism
gene wecD, expression of N-terminal fusion protein with a noncleavable His6-tag in Escherichia coli strain BL21(DE3)
Escherichia coli
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
acetyl-CoA
-
Escherichia coli
Crystallization (Commentary) (protein specific)
Crystallization
Organism
purified recombinant N-terminally His6-tagged WecD free and in complex with acetyl-CoA, hanging drop vapour diffusion method, mixing of 0.001 ml of 7.1 mg/ml protein in a 20 mM Tris-Cl, pH 8.0, 450 mM NaCl, and 5 mM DTT, with 0.001 ml reservoir solution aontaining 100 mM Na-acetate, pH 4.5, 10 mM ZnSO4, 20°C, 1 day, X-ray diffraction structure determination and analysis at 1.66-1.95 A resolution
Escherichia coli
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
Escherichia coli
-
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
Escherichia coli CFT073
-
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant N-terminally His6-tagged WecD from Escherichia coli strain BL21(DE3)
Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
-
719706
Escherichia coli
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
-
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
acetyl-CoA binding site structure, overview
719706
Escherichia coli
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
-
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
-
719706
Escherichia coli CFT073
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
-
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-galactose
acetyl-CoA binding site structure, overview
719706
Escherichia coli CFT073
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-galactose
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
WecD is a dimeric enzyme, with each monomer adopting the GNAT N-acetyltransferase fold, gel filtration and dynamic light scattering, three-dimensional structure comparison, overview
Escherichia coli
General Information
General Information
Commentary
Organism
physiological function
the enzyme is involved in biosynthesis of enterobacterial common antigen, ECA, a polysaccharide found on the outer membrane of virtually all Gram-negative enteric bacteria, that consists of three sugars, N-acetyl-D-glucosamine, N-acetyl-D-mannosaminuronic acid, and 4-acetamido-4,6-dideoxy-D-galactose, organized into trisaccharide repeating units having the sequence ->3)-alpha-D-Fuc4NAc-(1->4)-beta-D-ManNAcA-(1->4)-alpha-D-GlcNAc-(1->
Escherichia coli
evolution
WecD is a GNAT family member. The GNAT N-acetyltransferase fold is common to a number of enzymes involved in acetylation of histones, aminoglycoside antibiotics, serotonin, and sugars
Escherichia coli
additional information
usage of flexible docking to generate a WecD-bound model of the acetyl-CoATDP-fucosamine tetrahedral intermediate, representing the structure during acetyl transfer. that WecD does not possess a residue that directly functions as a catalytic base, although Tyr208 is well positioned to function as a general acid by protonating the thiolate anion of CoA, modeling of TDP-D-Fuc4N:acetyl-CoA transition state bound to WecD, overview. The WecD tetrahedral intermediate results from nucleophilic attack of the 4-amino group of TDP-fucosamine at the acetyl-CoA thioester carbon atom
Escherichia coli
General Information (protein specific)
General Information
Commentary
Organism
physiological function
the enzyme is involved in biosynthesis of enterobacterial common antigen, ECA, a polysaccharide found on the outer membrane of virtually all Gram-negative enteric bacteria, that consists of three sugars, N-acetyl-D-glucosamine, N-acetyl-D-mannosaminuronic acid, and 4-acetamido-4,6-dideoxy-D-galactose, organized into trisaccharide repeating units having the sequence ->3)-alpha-D-Fuc4NAc-(1->4)-beta-D-ManNAcA-(1->4)-alpha-D-GlcNAc-(1->
Escherichia coli
evolution
WecD is a GNAT family member. The GNAT N-acetyltransferase fold is common to a number of enzymes involved in acetylation of histones, aminoglycoside antibiotics, serotonin, and sugars
Escherichia coli
additional information
usage of flexible docking to generate a WecD-bound model of the acetyl-CoATDP-fucosamine tetrahedral intermediate, representing the structure during acetyl transfer. that WecD does not possess a residue that directly functions as a catalytic base, although Tyr208 is well positioned to function as a general acid by protonating the thiolate anion of CoA, modeling of TDP-D-Fuc4N:acetyl-CoA transition state bound to WecD, overview. The WecD tetrahedral intermediate results from nucleophilic attack of the 4-amino group of TDP-fucosamine at the acetyl-CoA thioester carbon atom
Escherichia coli
Other publictions for EC 2.3.1.210
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
719706
Hung
Crystal structure of TDP-fucos ...
Escherichia coli, Escherichia coli CFT073
J. Bacteriol.
188
5606-5617
2006
-
-
1
1
-
-
-
-
-
-
-
2
-
6
-
-
1
-
-
-
-
-
4
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
4
1
-
-
-
-
-
-
-
-
-
3
3
-
-
-