BRENDA - Enzyme Database
show all sequences of 2.3.1.209

Genetic analysis of genes involved in synthesis of modified 4-amino-4,6-dideoxyglucose in flagellin of Pseudomonas syringae pv. tabaci

Nguyen, L.C.; Yamamoto, M.; Ohnishi-Kameyama, M.; Andi, S.; Taguchi, F.; Iwaki, M.; Yoshida, M.; Ishii, T.; Konishi, T.; Tsunemi, K.; Ichinose, Y.; Mol. Genet. Genomics 282, 595-605 (2009)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene vioB, DNA and amino acid sequence determination and analysis
Pseudomonas syringae
Engineering
Amino acid exchange
Commentary
Organism
additional information
construction of a DELTAvioB deletion mutant strain
Pseudomonas syringae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
Pseudomonas syringae
-
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
Pseudomonas syringae 6605
-
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas syringae
C4B8D3
pv. tabaci, gene vioB
-
Pseudomonas syringae 6605
C4B8D3
pv. tabaci, gene vioB
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
-
720475
Pseudomonas syringae
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
i.e. dTDP-viosamine
720475
Pseudomonas syringae
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
i.e. dTDP-N-acetylviosamine
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
-
720475
Pseudomonas syringae 6605
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
i.e. dTDP-viosamine
720475
Pseudomonas syringae 6605
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
i.e. dTDP-N-acetylviosamine
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
acetyl-CoA
-
Pseudomonas syringae
Cloned(Commentary) (protein specific)
Commentary
Organism
gene vioB, DNA and amino acid sequence determination and analysis
Pseudomonas syringae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
acetyl-CoA
-
Pseudomonas syringae
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
additional information
construction of a DELTAvioB deletion mutant strain
Pseudomonas syringae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
Pseudomonas syringae
-
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
Pseudomonas syringae 6605
-
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
-
720475
Pseudomonas syringae
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
i.e. dTDP-viosamine
720475
Pseudomonas syringae
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
i.e. dTDP-N-acetylviosamine
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
-
720475
Pseudomonas syringae 6605
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
-
-
-
?
acetyl-CoA + dTDP-4-amino-4,6-dideoxy-alpha-D-glucose
i.e. dTDP-viosamine
720475
Pseudomonas syringae 6605
CoA + dTDP-4-acetamido-4,6-dideoxy-alpha-D-glucose
i.e. dTDP-N-acetylviosamine
-
-
?
General Information
General Information
Commentary
Organism
additional information
a vioB deletion mutant shows affected swimming and swarming motilities, adhesion ability, and virulence, phenotype, overview. Flagellins from DELTAvioA, DELTAvioB, and DELTAvioT mutants contain no viosamine residues but rhamnose chains of mixed lengths
Pseudomonas syringae
physiological function
glycosylation of Xagellin contributes to swimming and swarming motilities, adhesion ability, and consequently virulence with glycan structure at position Ser 201 harboring two L-rhamnoses and one modified 4-amino-4,6-dideoxyglucose, i.e. viosamine. Genes vioA, vioB, and vioT are essential for glycosylation of flagellin, and accordingly are required for bacterial virulence, e.g. on Nicotiana tabacum
Pseudomonas syringae
General Information (protein specific)
General Information
Commentary
Organism
additional information
a vioB deletion mutant shows affected swimming and swarming motilities, adhesion ability, and virulence, phenotype, overview. Flagellins from DELTAvioA, DELTAvioB, and DELTAvioT mutants contain no viosamine residues but rhamnose chains of mixed lengths
Pseudomonas syringae
physiological function
glycosylation of Xagellin contributes to swimming and swarming motilities, adhesion ability, and consequently virulence with glycan structure at position Ser 201 harboring two L-rhamnoses and one modified 4-amino-4,6-dideoxyglucose, i.e. viosamine. Genes vioA, vioB, and vioT are essential for glycosylation of flagellin, and accordingly are required for bacterial virulence, e.g. on Nicotiana tabacum
Pseudomonas syringae
Other publictions for EC 2.3.1.209
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
720475
Nguyen
Genetic analysis of genes invo ...
Pseudomonas syringae, Pseudomonas syringae 6605
Mol. Genet. Genomics
282
595-605
2009
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2
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687399
Wang
Biochemical characterization o ...
Escherichia coli, Escherichia coli O7, Shigella dysenteriae
J. Bacteriol.
189
8626-8635
2007
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2
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2
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6
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