BRENDA - Enzyme Database show
show all sequences of 2.3.1.174

Degradation of aromatics and chloroaromatics by Pseudomonas sp. strain B13: cloning, characterization, and analysis of sequences encoding 3-oxoadipate:succinyl-coenzyme A (CoA) transferase and 3-oxoadipyl-CoA thiolase

Gobel, M.; Kassel-Cati, K.; Schmidt, E.; Reineke, W.; J. Bacteriol. 184, 216-223 (2002)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41678
-
x * 41678, calculation from nucleotide sequence
Pseudomonas sp.
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CoA + 3-oxoadipyl-CoA
Pseudomonas sp.
3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates
succinyl-CoA + acetyl-CoA
-
-
?
CoA + 3-oxoadipyl-CoA
Pseudomonas sp. B13
3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates
succinyl-CoA + acetyl-CoA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. B13
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CoA + 3-oxoadipyl-CoA
3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates
646001
Pseudomonas sp.
succinyl-CoA + acetyl-CoA
-
-
-
?
CoA + 3-oxoadipyl-CoA
3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates
646001
Pseudomonas sp. B13
succinyl-CoA + acetyl-CoA
-
-
-
?
Subunits
Subunits
Commentary
Organism
?
x * 41678, calculation from nucleotide sequence
Pseudomonas sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
41678
-
x * 41678, calculation from nucleotide sequence
Pseudomonas sp.
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
CoA + 3-oxoadipyl-CoA
Pseudomonas sp.
3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates
succinyl-CoA + acetyl-CoA
-
-
?
CoA + 3-oxoadipyl-CoA
Pseudomonas sp. B13
3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates
succinyl-CoA + acetyl-CoA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
CoA + 3-oxoadipyl-CoA
3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates
646001
Pseudomonas sp.
succinyl-CoA + acetyl-CoA
-
-
-
?
CoA + 3-oxoadipyl-CoA
3-oxoadipyl-CoA thiolase carries out the ultimate step in the conversion of benzoate and 3-chlorobenzoate to tricarboxylic acid cycle intermediates
646001
Pseudomonas sp. B13
succinyl-CoA + acetyl-CoA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 41678, calculation from nucleotide sequence
Pseudomonas sp.
Other publictions for EC 2.3.1.174
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
704325
Marin
Modified 3-oxoadipate pathway ...
Pseudomonas reinekei
J. Bacteriol.
192
1543-1552
2010
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1
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1
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1
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1
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1
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1
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1
-
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-
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-
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-
-
-
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-
646001
Gobel
Degradation of aromatics and c ...
Pseudomonas sp., Pseudomonas sp. B13
J. Bacteriol.
184
216-223
2002
-
-
1
-
-
-
-
-
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1
2
-
24
-
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-
-
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2
1
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-
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-
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1
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1
2
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2
1
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-
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-
-
-
-
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-
-
-
-
646002
Kaschabek
Degradation of aromatics and c ...
Pseudomonas sp., Pseudomonas sp. B13
J. Bacteriol.
184
207-215
2002
-
-
-
-
-
-
5
2
-
-
2
-
-
23
-
-
1
-
-
-
1
-
4
1
-
-
-
2
1
-
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-
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-
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-
5
-
2
-
-
2
-
-
-
-
1
-
-
1
-
4
1
-
-
-
2
1
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-
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