Literature summary for 2.3.1.160 extracted from
Stoeckigt, J.; Panjikar, S.; Ruppert, M.; Barleben, L.; Ma, X.; Loris, E.; Hill, M.
The molecular architecture of major enzymes from ajmaline biosynthetic pathway (2007), Phytochem. Rev., 6, 15-34.
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
expression in Escherichia coli |
Rauvolfia serpentina |
Crystallization (Commentary)
Crystallization (Comment) |
Organism |
using the hanging-drop vapour-diffusion method |
Rauvolfia serpentina |
Protein Variants
Protein Variants |
Comment |
Organism |
D164A |
reduction in enzyme activity |
Rauvolfia serpentina |
D32A |
reduction in enzyme activity |
Rauvolfia serpentina |
D362A |
reduction in enzyme activity |
Rauvolfia serpentina |
H160A |
reduction in enzyme activity |
Rauvolfia serpentina |
S29A |
reduction in enzyme activity |
Rauvolfia serpentina |
Organism
Organism |
UniProt |
Comment |
Textmining |
Rauvolfia serpentina |
- |
- |
- |
Purification (Commentary)
Purification (Comment) |
Organism |
of the recombinant protein by his-tag affinity column chromatography |
Rauvolfia serpentina |
Substrates and Products (Substrate)
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
16-epi-vellosimine + acetyl-CoA |
VS responsible for generation of the basic carbon skeleton of the target compound ajmaline, His160 and Asp164 are the most important amino acids for the catalytic process, His-X-X-X-Asp sequence together with Asp-Phe-Gly-Trp-Gly are highly conserved motifs in acyltransferases |
Rauvolfia serpentina |
vinorine + CoA |
- |
? |
|
Synonyms
Synonyms |
Comment |
Organism |
vinorine synthase |
a member of the BAHD enzyme super-family, benzylalcohol acetyl-, anthocyanin-O-hydroxy-cinnamoyl-, anthranilate-N-hydroxy-cinnamoyl-benzoyl- and deacetylvindoline acetyl transferase, involved in synthesis of ajmaline |
Rauvolfia serpentina |
VS |
- |
Rauvolfia serpentina |