Literature summary for 2.3.1.15 extracted from

Niu, Y.F.; Wang, X.; Hu, D.X.; Balamurugan, S.; Li, D.W.; Yang, W.D.; Liu, J.S.; Li, H.Y.
Molecular characterization of a glycerol-3-phosphate acyltransferase reveals key features essential for triacylglycerol production in Phaeodactylum tricornutum (2016), Biotechnol. Biofuels, 9, 60 .

Cloned(Commentary)

Cloned (Comment)	Organism
enzyme cloning and overexpression in Phaeodactylum tricornutum strain CCMP2561, PtGPAT cDNA is fused between the fucoxanthin chlorophyll a/c binding protein (fcp) fcpC promoter and fcpA terminator of Phaeodactylum tricornutum. Furthermore, an Omega leader sequence is added before the coding region of PtGPAT for enhancing its translation. A c-Myc tag is also fused to the C-terminal of PtGPAT, quantitative real-time RT-PCR expression analysis	Phaeodactylum tricornutum

Cloned (Comment)

Organism

enzyme cloning and overexpression in Phaeodactylum tricornutum strain CCMP2561, PtGPAT cDNA is fused between the fucoxanthin chlorophyll a/c binding protein (fcp) fcpC promoter and fcpA terminator of Phaeodactylum tricornutum. Furthermore, an Omega leader sequence is added before the coding region of PtGPAT for enhancing its translation. A c-Myc tag is also fused to the C-terminal of PtGPAT, quantitative real-time RT-PCR expression analysis

Phaeodactylum tricornutum

Protein Variants

Protein Variants	Comment	Organism
additional information	enzyme overexpression in Phaeodactylum tricornutum leads to production of volumes of oil bodies and twofold increased neutral lipid content. Fatty acid composition is analyzed by GC-MS, which shows significantly higher proportion of unsaturated fatty acids compared to wild-type	Phaeodactylum tricornutum

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
chloroplast	-	Phaeodactylum tricornutum	9507	-
additional information	subcellular localization analysis, overview	Phaeodactylum tricornutum	-	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
acyl-CoA + sn-glycerol 3-phosphate	Phaeodactylum tricornutum	-	CoA + 1-acyl-sn-glycerol 3-phosphate	-	?
acyl-CoA + sn-glycerol 3-phosphate	Phaeodactylum tricornutum CCAP 1055/1	-	CoA + 1-acyl-sn-glycerol 3-phosphate	-	?

Organism

Organism	UniProt	Comment	Textmining
Phaeodactylum tricornutum	B7FRB8	-	-
Phaeodactylum tricornutum CCAP 1055/1	B7FRB8	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
acyl-CoA + sn-glycerol 3-phosphate	-	Phaeodactylum tricornutum	CoA + 1-acyl-sn-glycerol 3-phosphate	-	?
acyl-CoA + sn-glycerol 3-phosphate	-	Phaeodactylum tricornutum CCAP 1055/1	CoA + 1-acyl-sn-glycerol 3-phosphate	-	?

Synonyms

Synonyms	Comment	Organism
glycerol-3-phosphate acyltransferase	-	Phaeodactylum tricornutum
GPAT	-	Phaeodactylum tricornutum
gpt1	-	Phaeodactylum tricornutum
PHATRDRAFT_42446	-	Phaeodactylum tricornutum
PtGPAT	-	Phaeodactylum tricornutum

General Information

General Information	Comment	Organism
malfunction	changes in fatty acid composition regulated by overexpression of GPAT, changes in fatty acid composition in transgenic and wild-type microalgae, overview	Phaeodactylum tricornutum
metabolism	glycerol-3-phosphate acyltransferase (GPAT) is the critical enzyme that catalyzes the first step of triacylglycerol (TAG) formation, it is involved in the lipid metabolism of the diatom	Phaeodactylum tricornutum