General Stability | Organism |
---|---|
all operation involving retinoids are performed at darkness, or under red light | Rattus norvegicus |
all operation involving retinoids are performed at darkness, or under red light | Bos taurus |
buffers are bubbled with argon prior to use | Bos taurus |
dithiothreitol stabilizes | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.002 | - |
all-trans-retinol | - |
Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Rattus norvegicus | - |
- |
microsome | - |
Bos taurus | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
intestinal mucosa | - |
Rattus norvegicus | - |
liver | - |
Rattus norvegicus | - |
mammary gland | - |
Rattus norvegicus | - |
retinal pigment epithelium | - |
Bos taurus | - |
testis | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.00003 | - |
intestinal LRAT | Rattus norvegicus |
0.00006 | - |
mammary gland LRAT | Rattus norvegicus |
0.000094 | - |
testis LRAT | Rattus norvegicus |
0.000145 | - |
liver LRAT | Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, 10 mM Tris acetate, pH 7, 1 mM dithiothreitol, microsomal fraction, stable for at least 6 months | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | little or no ability to transfer acyl groups from lysophosphatidylcholine, phosphatidylethanolamine or phosphatidic acid to retinol-(cellular-retinol-binding protein) | Rattus norvegicus | ? | - |
? | |
additional information | the fatty acid in the 2-position is important in substrate recognition | Rattus norvegicus | ? | - |
? | |
additional information | the fatty acid in the 2-position is important in substrate recognition | Bos taurus | ? | - |
? | |
phosphatidylcholine + retinol-(cellular-retinol-binding-protein) | - |
Rattus norvegicus | 2-acylglycerophosphocholine + retinyl ester-(cellular-retinol-binding-protein) | - |
? | |
phosphatidylcholine + retinol-(cellular-retinol-binding-protein) | - |
Bos taurus | 2-acylglycerophosphocholine + retinyl ester-(cellular-retinol-binding-protein) | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Rattus norvegicus |
37 | - |
assay at | Bos taurus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
half-life in absence of substrate: 17 min | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Bos taurus |