Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
48000 | - |
gel filtration | Ocimum basilicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + shikimate | Ocimum basilicum | enzyme appears to play a critical role in the phenylpropanoid pathway, both upstream and downstream of the 3-hydroxylation step | CoA + 5-O-(4-coumaroyl)shikimate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ocimum basilicum | - |
SW and EMX-1 | - |
Purification (Comment) | Organism |
---|---|
partial | Ocimum basilicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
gland | - |
Ocimum basilicum | - |
leaf | - |
Ocimum basilicum | - |
additional information | enzyme activity is much higher in tissues that are actively producing eugenol than in tissues that are not | Ocimum basilicum | - |
Storage Stability | Organism |
---|---|
incubation for days at room temperature, multiple freeze/thaw cycles, holding at -20°C for several months, with or without the addition of 10% glycerol, no loss of activity | Ocimum basilicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-coumaroyl-CoA + shikimate | - |
Ocimum basilicum | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
4-coumaroyl-CoA + shikimate | enzyme appears to play a critical role in the phenylpropanoid pathway, both upstream and downstream of the 3-hydroxylation step | Ocimum basilicum | CoA + 5-O-(4-coumaroyl)shikimate | - |
? | |
caffeoyl-CoA + shikimate | the activity is 100fold lower than the activity with coumaroyl-CoA | Ocimum basilicum | CoA + 5-O-caffeoylshikimate | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
enzyme extremely stable | Ocimum basilicum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
above 40°C rapid and irreversible loss of activity | Ocimum basilicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
50 mM potassium phosphate buffer | Ocimum basilicum |