BRENDA - Enzyme Database show
show all sequences of 2.3.1.118

N-Hydroxyarylamine O-acetyltransferase in hamster liver: identity with arylhydroxamic acid N,O-acetyltransferase and arylamine N-acetyltransferase

Saito, K.; Shinohara, A.; Kamataki, T.; Kato, R.; J. Biochem. 99, 1689-1697 (1986)

Data extracted from this reference:

General Stability
General Stability
Organism
glycerol 50% v/v, stabilizes during storage
Mesocricetus auratus
Inhibitors
Inhibitors
Commentary
Organism
Structure
1-Nitro-2-naphthol
-
Mesocricetus auratus
2-Aminofluorene
-
Mesocricetus auratus
iodoacetamide
-
Mesocricetus auratus
additional information
thiolactomycin does not inhibit enzyme from Syrian golden hamster
Mesocricetus auratus
Pentachlorophenol
-
Mesocricetus auratus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.27
-
N-Hydroxy-2-acetylaminofluorene
-
Mesocricetus auratus
0.37
-
acetyl-CoA
reaction of O-acetyl transfer to 2-hydroxyamino-6-methyldipyrido[1,2-alpha: 3',2'-d]imidazole
Mesocricetus auratus
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mesocricetus auratus
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
gel filtration
Mesocricetus auratus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + an N-hydroxyarylamine
Mesocricetus auratus
involved in mutagenic, metabolic activation of carcinogenic arylamines to form DNA-binding species
CoA + an N-acetoxyarylamine
-
Mesocricetus auratus
?
acetyl-CoA + an N-hydroxyarylamine
Mesocricetus auratus
the enzyme from liver, but not from bacteria, can also catalyse acetylation of arylamines and N,O-acetylation of arylhydroxamates
CoA + an N-acetoxyarylamine
-
Mesocricetus auratus
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mesocricetus auratus
-
Syrian golden hamster
-
Purification (Commentary)
Commentary
Organism
copurifies with arylamine acetyltransferase and arylhydroxamic acid N,O-acetyltransferase; purification by ammonium sulfate followed by DEAE cellulose, gel filtration on GCL-2000 and HPKB-hydroxyapatite chromatography
Mesocricetus auratus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
liver
-
Mesocricetus auratus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.82
-
2-hydroxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole as substrate
Mesocricetus auratus
Storage Stability
Storage Stability
Organism
-80°C, glycerol 50% v/v, stabilizes during storage
Mesocricetus auratus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
486155
Mesocricetus auratus
CoA + N-acetoxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
486155
Mesocricetus auratus
?
acetyl-CoA + 3-hydroxyamino-1-methyl-pyrido[4,3-b]-indole
-
486155
Mesocricetus auratus
CoA + N-acetoxy-amino-1-methyl-pyrido[4,3-b]-indole
-
486155
Mesocricetus auratus
?
acetyl-CoA + an N-hydroxyarylamine
involved in mutagenic, metabolic activation of carcinogenic arylamines to form DNA-binding species
486155
Mesocricetus auratus
CoA + an N-acetoxyarylamine
-
486155
Mesocricetus auratus
?
acetyl-CoA + an N-hydroxyarylamine
the enzyme from liver, but not from bacteria, can also catalyse acetylation of arylamines and N,O-acetylation of arylhydroxamates
486155
Mesocricetus auratus
CoA + an N-acetoxyarylamine
-
486155
Mesocricetus auratus
?
acetyl-CoA + N-hydroxy-2-aminofluorene
-
486155
Mesocricetus auratus
CoA + N-acetoxy-2-aminofluorene
-
486155
Mesocricetus auratus
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 33000
Mesocricetus auratus
General Stability (protein specific)
General Stability
Organism
glycerol 50% v/v, stabilizes during storage
Mesocricetus auratus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1-Nitro-2-naphthol
-
Mesocricetus auratus
2-Aminofluorene
-
Mesocricetus auratus
iodoacetamide
-
Mesocricetus auratus
additional information
thiolactomycin does not inhibit enzyme from Syrian golden hamster
Mesocricetus auratus
Pentachlorophenol
-
Mesocricetus auratus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.27
-
N-Hydroxy-2-acetylaminofluorene
-
Mesocricetus auratus
0.37
-
acetyl-CoA
reaction of O-acetyl transfer to 2-hydroxyamino-6-methyldipyrido[1,2-alpha: 3',2'-d]imidazole
Mesocricetus auratus
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Mesocricetus auratus
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
gel filtration
Mesocricetus auratus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
acetyl-CoA + an N-hydroxyarylamine
Mesocricetus auratus
involved in mutagenic, metabolic activation of carcinogenic arylamines to form DNA-binding species
CoA + an N-acetoxyarylamine
-
Mesocricetus auratus
?
acetyl-CoA + an N-hydroxyarylamine
Mesocricetus auratus
the enzyme from liver, but not from bacteria, can also catalyse acetylation of arylamines and N,O-acetylation of arylhydroxamates
CoA + an N-acetoxyarylamine
-
Mesocricetus auratus
?
Purification (Commentary) (protein specific)
Commentary
Organism
copurifies with arylamine acetyltransferase and arylhydroxamic acid N,O-acetyltransferase; purification by ammonium sulfate followed by DEAE cellulose, gel filtration on GCL-2000 and HPKB-hydroxyapatite chromatography
Mesocricetus auratus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
liver
-
Mesocricetus auratus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.82
-
2-hydroxyamino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole as substrate
Mesocricetus auratus
Storage Stability (protein specific)
Storage Stability
Organism
-80°C, glycerol 50% v/v, stabilizes during storage
Mesocricetus auratus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
acetyl-CoA + 2-hydroxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
486155
Mesocricetus auratus
CoA + N-acetoxy-amino-6-methyldipyrido[1,2-a: 3',2'-d]imidazole
-
486155
Mesocricetus auratus
?
acetyl-CoA + 3-hydroxyamino-1-methyl-pyrido[4,3-b]-indole
-
486155
Mesocricetus auratus
CoA + N-acetoxy-amino-1-methyl-pyrido[4,3-b]-indole
-
486155
Mesocricetus auratus
?
acetyl-CoA + an N-hydroxyarylamine
involved in mutagenic, metabolic activation of carcinogenic arylamines to form DNA-binding species
486155
Mesocricetus auratus
CoA + an N-acetoxyarylamine
-
486155
Mesocricetus auratus
?
acetyl-CoA + an N-hydroxyarylamine
the enzyme from liver, but not from bacteria, can also catalyse acetylation of arylamines and N,O-acetylation of arylhydroxamates
486155
Mesocricetus auratus
CoA + an N-acetoxyarylamine
-
486155
Mesocricetus auratus
?
acetyl-CoA + N-hydroxy-2-aminofluorene
-
486155
Mesocricetus auratus
CoA + N-acetoxy-2-aminofluorene
-
486155
Mesocricetus auratus
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 33000
Mesocricetus auratus
Other publictions for EC 2.3.1.118
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
729708
Zhang
Reversibly acetylated lysine r ...
Escherichia coli
FEBS J.
280
1966-1979
2013
-
-
1
-
4
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658532
Winter
Identification of cytochrome P ...
Homo sapiens
Drug Metab. Dispos.
33
969-976
2005
-
-
-
-
-
-
-
5
1
-
-
-
-
3
-
-
-
-
-
1
4
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
1
-
-
-
-
-
-
-
-
1
4
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
659970
Summerscales
Human acetyl CoA:arylamine N-a ...
Homo sapiens
Mol. Pharmacol.
65
220-226
2004
-
-
-
-
5
-
-
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486161
Yamamura
Purification and biochemical p ...
Escherichia coli
Biochim. Biophys. Acta
1475
10-16
2000
-
-
1
-
-
-
10
10
-
-
2
1
-
2
-
-
1
-
-
1
10
1
11
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
10
-
10
-
-
2
1
-
-
-
1
-
1
10
1
11
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
486165
Espinosa-Aguirre
New O-acetyltransferase defici ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium TA100, Salmonella enterica subsp. enterica serovar Typhimurium TA98
Mutat. Res.
439
159-169
1999
-
-
1
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486163
Sinclair
Purification, characterization ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium LT2
Protein Expr. Purif.
12
371-380
1998
-
-
-
1
-
-
-
3
-
-
1
-
-
44
-
-
1
-
-
-
1
-
6
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
1
-
-
1
-
6
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
486162
Hein
Metabolic activation of N-hydr ...
Homo sapiens
Cancer Res.
55
3531-3536
1995
-
-
1
-
-
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
1
1
-
3
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
486164
Land
Purification and characterizat ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley
Carcinogenesis
14
1441-1449
1993
-
-
-
-
-
-
-
-
1
-
1
-
-
162
-
-
1
-
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
1
-
1
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486157
Watanabe
Involvement of Cys69 residue i ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium TA 1538, Salmonella enterica subsp. enterica serovar Typhimurium TA98
J. Biol. Chem.
267
8429-8436
1992
-
-
1
-
2
-
-
1
1
-
1
-
-
12
-
-
1
-
-
-
4
-
3
1
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
1
1
-
1
-
-
-
-
1
-
-
4
-
3
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
487133
Hein
Acetylator genotype and arylam ...
Homo sapiens, Mesocricetus auratus, Mus musculus, Mus musculus C57/BL6J, Oryctolagus cuniculus, Salmonella enterica subsp. enterica serovar Typhimurium
Biochim. Biophys. Acta
948
37-66
1988
-
-
-
-
-
-
-
-
4
-
1
10
-
65
-
-
2
-
-
17
6
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
4
-
1
10
-
-
-
2
-
17
6
-
18
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486155
Saito
N-Hydroxyarylamine O-acetyltra ...
Mesocricetus auratus
J. Biochem.
99
1689-1697
1986
-
-
-
-
-
1
5
2
1
-
1
2
-
1
-
-
1
-
-
1
1
1
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
2
1
-
1
2
-
-
-
1
-
1
1
1
5
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486158
Saito
A new assay for N-hydroxyaryla ...
Rattus norvegicus, Rattus norvegicus Sprague-Dawley, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium TA98
Anal. Biochem.
152
226-231
1986
1
-
-
-
-
-
4
1
-
-
-
-
-
167
-
-
1
-
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
4
-
1
-
-
-
-
-
-
-
1
-
1
-
-
4
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
486156
Saito
Metabolic activation of mutage ...
Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium TA98
Arch. Biochem. Biophys.
239
286-295
1985
-
-
-
-
-
-
9
1
-
-
1
3
-
6
-
-
1
-
-
-
1
1
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
9
-
1
-
-
1
3
-
-
-
1
-
-
1
1
16
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486160
Glowinski
Evidence that arylhydroxamic a ...
Oryctolagus cuniculus
J. Biol. Chem.
255
7883-7890
1980
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
1
-
-
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-