BRENDA - Enzyme Database show
show all sequences of 2.3.1.110

Substrate promiscuity of a rosmarinic acid synthase from lavender (Lavandula angustifolia L.)

Landmann, C.; Huecherig, S.; Fink, B.; Hoffmann, T.; Dittlein, D.; Coiner, H.A.; Schwab, W.; Planta 234, 305-320 (2011)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
AAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree of BAHD acyltransferases, expression of GST-tagged AAT1 in Escherichia coli strain Rosetta(DE3)pLysS
Lavandula angustifolia
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
tyramine
with caffeoyl-CoA, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.018
-
tyramine
with cinnamoyl-CoA, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.036
-
tyramine
with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.057
-
4-Coumaroyl-CoA
with tyramine, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.112
-
phenethylamine
with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.157
-
4-Coumaroyl-CoA
with tryptamine, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.192
-
feruloyl-CoA
with tyramine, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.243
-
tyramine
with feruloyl-CoA, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.3
-
4-Coumaroyl-CoA
with phenethylamine, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.326
-
caffeoyl-CoA
with tyramine, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.49
-
cinnamoyl-CoA
with tyramine, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Lavandula angustifolia
5829
-
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50600
-
1 * 50600, about, LAAT1, sequence determination
Lavandula angustifolia
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Lavandula angustifolia
recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Lavandula angustifolia
Q0GA14
-
-
Purification (Commentary)
Commentary
Organism
recombinant GST-tagged AAT1 from Escherichia coli by cell disruption through freeze-thaw cycles, and glutathione affinity chromatography
Lavandula angustifolia
Source Tissue
Source Tissue
Commentary
Organism
Textmining
flower
-
Lavandula angustifolia
-
Storage Stability
Storage Stability
Organism
4°C, purified recombinant GST-tagged AAT1, in 50 mM Tris-Cl, pH 8.0, and 10 mM reduced glutathione, 4 weeks, stable
Lavandula angustifolia
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + phenethylamine
-
720789
Lavandula angustifolia
CoA + N-(4-coumaroyl)phenethylamine
-
-
-
?
4-coumaroyl-CoA + tryptamine
-
720789
Lavandula angustifolia
CoA + N-(4-coumaroyl)tryptamine
-
-
-
?
4-coumaroyl-CoA + tyramine
-
720789
Lavandula angustifolia
CoA + N-(4-coumaroyl)tyramine
-
-
-
?
caffeoyl-CoA + tyramine
-
720789
Lavandula angustifolia
CoA + N-caffeoyltyramine
-
-
-
?
cinnamoyl-CoA + tyramine
-
720789
Lavandula angustifolia
CoA + N-cinnamoyltyramine
-
-
-
?
feruloyl-CoA + tyramine
-
720789
Lavandula angustifolia
CoA + N-feruloyltyramine
-
-
-
?
additional information
recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity
720789
Lavandula angustifolia
?
-
-
-
-
additional information
substrate specificity of recombinant AAT1, overview. No or poor activity with anthranilic acid
720789
Lavandula angustifolia
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 50600, about, LAAT1, sequence determination
Lavandula angustifolia
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Lavandula angustifolia
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Lavandula angustifolia
Cloned(Commentary) (protein specific)
Commentary
Organism
AAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree of BAHD acyltransferases, expression of GST-tagged AAT1 in Escherichia coli strain Rosetta(DE3)pLysS
Lavandula angustifolia
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.005
-
tyramine
with caffeoyl-CoA, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.018
-
tyramine
with cinnamoyl-CoA, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.036
-
tyramine
with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.057
-
4-Coumaroyl-CoA
with tyramine, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.112
-
phenethylamine
with 4-coumaroyl-CoA, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.157
-
4-Coumaroyl-CoA
with tryptamine, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.192
-
feruloyl-CoA
with tyramine, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.243
-
tyramine
with feruloyl-CoA, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.3
-
4-Coumaroyl-CoA
with phenethylamine, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.326
-
caffeoyl-CoA
with tyramine, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
0.49
-
cinnamoyl-CoA
with tyramine, pH 7.5, 30°C, recombinant AAT1
Lavandula angustifolia
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Lavandula angustifolia
5829
-
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
50600
-
1 * 50600, about, LAAT1, sequence determination
Lavandula angustifolia
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Lavandula angustifolia
recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant GST-tagged AAT1 from Escherichia coli by cell disruption through freeze-thaw cycles, and glutathione affinity chromatography
Lavandula angustifolia
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
flower
-
Lavandula angustifolia
-
Storage Stability (protein specific)
Storage Stability
Organism
4°C, purified recombinant GST-tagged AAT1, in 50 mM Tris-Cl, pH 8.0, and 10 mM reduced glutathione, 4 weeks, stable
Lavandula angustifolia
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + phenethylamine
-
720789
Lavandula angustifolia
CoA + N-(4-coumaroyl)phenethylamine
-
-
-
?
4-coumaroyl-CoA + tryptamine
-
720789
Lavandula angustifolia
CoA + N-(4-coumaroyl)tryptamine
-
-
-
?
4-coumaroyl-CoA + tyramine
-
720789
Lavandula angustifolia
CoA + N-(4-coumaroyl)tyramine
-
-
-
?
caffeoyl-CoA + tyramine
-
720789
Lavandula angustifolia
CoA + N-caffeoyltyramine
-
-
-
?
cinnamoyl-CoA + tyramine
-
720789
Lavandula angustifolia
CoA + N-cinnamoyltyramine
-
-
-
?
feruloyl-CoA + tyramine
-
720789
Lavandula angustifolia
CoA + N-feruloyltyramine
-
-
-
?
additional information
recombinant LaAAT1 exhibits a broad substrate tolerance accepting (hydroxy)cinnamoyl-CoAs as acyl donors and not only tyramine, tryptamine, phenylethylamine, and anthranilic acid but also shikimic acid and 4-hydroxyphenyllactic acid as acceptors, showing substrate promiscuity
720789
Lavandula angustifolia
?
-
-
-
-
additional information
substrate specificity of recombinant AAT1, overview. No or poor activity with anthranilic acid
720789
Lavandula angustifolia
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 50600, about, LAAT1, sequence determination
Lavandula angustifolia
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Lavandula angustifolia
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Lavandula angustifolia
Other publictions for EC 2.3.1.110
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736840
Campos
Transgenic tomato plants overe ...
Solanum lycopersicum
Mol. Plant Microbe Interact.
27
1159-1169
2014
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1
1
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720789
Landmann
Substrate promiscuity of a ros ...
Lavandula angustifolia
Planta
234
305-320
2011
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1
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11
1
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1
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702823
Kang
Production of plant-specific t ...
Capsicum annuum
Biotechnol. Lett.
31
1469-1475
2009
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702831
Park
Endosperm-specific expression ...
Capsicum annuum
Biotechnol. Lett.
31
911-915
2009
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1
1
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1
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4
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1
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673815
Niehl
-
Systemic Potato virus X infect ...
Solanum tuberosum
Funct. Plant Biol.
33
593-604
2006
-
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676598
Kang
Functional analysis of the ami ...
Capsicum annuum
Plant Physiol.
140
704-715
2006
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1
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3
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8
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1
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1
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1
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6
1
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1
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3
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8
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1
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6
1
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660324
Hagel
Elevated tyrosine decarboxylas ...
Nicotiana tabacum
Planta
221
904-914
2005
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1
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4
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660244
Jang
Production of coumaroylseroton ...
Capsicum annuum
Plant Physiol.
135
346-356
2004
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1
9
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3
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1
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7
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9
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7
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486128
Back
Cloning and characterization o ...
Capsicum annuum
Plant Cell Physiol.
42
475-481
2001
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1
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1
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5
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5
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5
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6
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486131
Negrel
L-Tyrosine beta-naphthylamide ...
Nicotiana tabacum, Solanum tuberosum
Phytochemistry
56
523-527
2001
-
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8
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4
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2
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2
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8
8
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2
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2
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486130
Ishihara
Induction of N-hydroxycinnamoy ...
Zea mays
Biosci. Biotechnol. Biochem.
64
1025-1031
2000
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2
8
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1
1
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7
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1
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7
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486129
Farmer
Identification and characteriz ...
Nicotiana tabacum
Eur. J. Biochem.
263
686-694
1999
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1
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3
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1
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3
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2
1
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1
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2
1
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486133
Schmidt
Cloning and expression of a po ...
Solanum tuberosum
J. Biol. Chem.
274
4273-4280
1999
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1
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2
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2
1
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4
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1
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9
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1
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1
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1
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9
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486134
Yu
Purification, characterization ...
Papaver somniferum
Planta
209
33-44
1999
-
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3
6
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2
1
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1
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1
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4
1
3
5
1
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1
1
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3
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6
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2
1
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1
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4
1
3
5
1
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1
1
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-
486132
Negrel
Purification, characterization ...
Nicotiana tabacum
Eur. J. Biochem.
247
1127-1135
1997
1
-
-
-
-
-
11
12
-
-
2
1
-
2
-
-
1
1
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1
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13
1
-
-
-
-
-
-
-
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9
-
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1
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-
-
-
-
-
-
11
9
12
-
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2
1
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-
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1
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-
1
-
13
1
-
-
-
-
-
-
-
-
-
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-
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486127
Fleurence
-
Partial purification of tyrami ...
Nicotiana tabacum
Phytochemistry
28
733-736
1989
-
-
-
-
-
2
2
4
-
-
-
1
-
1
1
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1
-
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1
1
2
6
-
1
-
-
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1
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-
-
-
-
-
-
-
-
-
-
-
2
-
2
-
4
-
-
-
1
-
1
-
1
-
1
1
2
6
-
1
-
-
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1
-
-
-
-
-
-
-
-
-
486126
Negrel
-
The biosynthesis of feruloylty ...
Nicotiana tabacum
Phytochemistry
23
2797-2801
1984
-
-
-
-
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1
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6
1
1
1
1
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1
1
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1
-
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1
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1
14
-
1
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-
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4
1
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-
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-
-
-
-
-
-
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1
-
-
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6
1
1
1
1
-
1
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1
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1
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1
14
-
1
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-
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4
1
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