BRENDA - Enzyme Database show
show all sequences of 2.3.1.110

Functional analysis of the amine substrate specificity domain of pepper tyramine and serotonin N-hydroxycinnamoyltransferases

Kang, S.; Kang, K.; Chung, G.C.; Choi, D.; Ishihara, A.; Lee, D.; Back, K.; Plant Physiol. 140, 704-715 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli
Capsicum annuum
Engineering
Amino acid exchange
Commentary
Organism
F145Y
KM-value for tyramine is 2.3fold lower than wild-type value, Vmax is 3fold lower than wild-type value. Wild-type enzyme shows no activity with serotonin, mutant enzyms shows activity with serotonin
Capsicum annuum
F145Y/Y147F
KM-value for tyramine is 2.3fold lower than wild-type value, Vmax is 4.2fold lower than wild-type value. Wild-type enzyme shows no activity with serotonin, mutant enzyms shows activity with serotonin
Capsicum annuum
additional information
a series of chimeric genes is constructed by reciprocal exchange of DNA segments between the serotonin N-hydroxycinnamoyltransferase and tyramine N-hydroxycinnamoyltransferase cDNAs. Functional characterization of the recombinant chimeric proteins revealed that the amino acid residues 129 to 165 of serotonin N-hydroxycinnamoyltransferase and the corresponding residues 125 to 160 in tyramine N-hydroxycinnamoyltransferase are critical structural determinants for amine substrate specificity
Capsicum annuum
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
4-Coumaroyl-CoA
30C, pH 8.5, recombinant enzyme
Capsicum annuum
0.04
-
tyramine
30C, pH 8.5, recombinant enzyme
Capsicum annuum
0.053
-
feruloyl-CoA
30C, pH 8.5, recombinant enzyme
Capsicum annuum
0.17
-
tyramine
30C, pH 8.5, mutant enzyme F145Y; 30C, pH 8.5, mutant enzyme F145Y/Y147F
Capsicum annuum
0.779
-
dopamine
30C, pH 8.5, recombinant enzyme
Capsicum annuum
20.98
-
serotonin
30C, pH 8.5, mutant enzyme F145Y
Capsicum annuum
24
-
tryptamine
30C, pH 8.5, recombinant enzyme
Capsicum annuum
25.44
-
serotonin
30C, pH 8.5, mutant enzyme F145Y/Y147F
Capsicum annuum
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28221
-
x * 28221, calculatred from nucleotide sequence
Capsicum annuum
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Capsicum annuum
Q5D8C0
-
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
THT mRNA is expressed constitutively in all pepper organs
Capsicum annuum
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + tyramine
-
676598
Capsicum annuum
CoA + N-(4-coumaroyl)tyramine
-
-
-
?
caffeoyl-CoA + serotonin
-
676598
Capsicum annuum
CoA + N-caffeoylserotonin
-
-
-
-
feruloyl-CoA + dopamine
-
676598
Capsicum annuum
CoA + feruloyldopamine
-
-
-
?
feruloyl-CoA + tryptamine
-
676598
Capsicum annuum
CoA + feruloyltryptamine
-
-
-
?
feruloyl-CoA + tyramine
-
676598
Capsicum annuum
CoA + feruloyltyramine
-
-
-
?
additional information
wild-type enzyme shows no activity with serotonin, mutant enzyms F145Y and F145Y/Y147F show activity with serotonin
676598
Capsicum annuum
?
-
-
-
-
Subunits
Subunits
Commentary
Organism
?
x * 28221, calculatred from nucleotide sequence
Capsicum annuum
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli
Capsicum annuum
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
F145Y
KM-value for tyramine is 2.3fold lower than wild-type value, Vmax is 3fold lower than wild-type value. Wild-type enzyme shows no activity with serotonin, mutant enzyms shows activity with serotonin
Capsicum annuum
F145Y/Y147F
KM-value for tyramine is 2.3fold lower than wild-type value, Vmax is 4.2fold lower than wild-type value. Wild-type enzyme shows no activity with serotonin, mutant enzyms shows activity with serotonin
Capsicum annuum
additional information
a series of chimeric genes is constructed by reciprocal exchange of DNA segments between the serotonin N-hydroxycinnamoyltransferase and tyramine N-hydroxycinnamoyltransferase cDNAs. Functional characterization of the recombinant chimeric proteins revealed that the amino acid residues 129 to 165 of serotonin N-hydroxycinnamoyltransferase and the corresponding residues 125 to 160 in tyramine N-hydroxycinnamoyltransferase are critical structural determinants for amine substrate specificity
Capsicum annuum
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
4-Coumaroyl-CoA
30C, pH 8.5, recombinant enzyme
Capsicum annuum
0.04
-
tyramine
30C, pH 8.5, recombinant enzyme
Capsicum annuum
0.053
-
feruloyl-CoA
30C, pH 8.5, recombinant enzyme
Capsicum annuum
0.17
-
tyramine
30C, pH 8.5, mutant enzyme F145Y; 30C, pH 8.5, mutant enzyme F145Y/Y147F
Capsicum annuum
0.779
-
dopamine
30C, pH 8.5, recombinant enzyme
Capsicum annuum
20.98
-
serotonin
30C, pH 8.5, mutant enzyme F145Y
Capsicum annuum
24
-
tryptamine
30C, pH 8.5, recombinant enzyme
Capsicum annuum
25.44
-
serotonin
30C, pH 8.5, mutant enzyme F145Y/Y147F
Capsicum annuum
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
28221
-
x * 28221, calculatred from nucleotide sequence
Capsicum annuum
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
THT mRNA is expressed constitutively in all pepper organs
Capsicum annuum
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4-coumaroyl-CoA + tyramine
-
676598
Capsicum annuum
CoA + N-(4-coumaroyl)tyramine
-
-
-
?
caffeoyl-CoA + serotonin
-
676598
Capsicum annuum
CoA + N-caffeoylserotonin
-
-
-
-
feruloyl-CoA + dopamine
-
676598
Capsicum annuum
CoA + feruloyldopamine
-
-
-
?
feruloyl-CoA + tryptamine
-
676598
Capsicum annuum
CoA + feruloyltryptamine
-
-
-
?
feruloyl-CoA + tyramine
-
676598
Capsicum annuum
CoA + feruloyltyramine
-
-
-
?
additional information
wild-type enzyme shows no activity with serotonin, mutant enzyms F145Y and F145Y/Y147F show activity with serotonin
676598
Capsicum annuum
?
-
-
-
-
Subunits (protein specific)
Subunits
Commentary
Organism
?
x * 28221, calculatred from nucleotide sequence
Capsicum annuum
Other publictions for EC 2.3.1.110
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
736840
Campos
Transgenic tomato plants overe ...
Solanum lycopersicum
Mol. Plant Microbe Interact.
27
1159-1169
2014
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5
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1
1
-
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-
720789
Landmann
Substrate promiscuity of a ros ...
Lavandula angustifolia
Planta
234
305-320
2011
-
-
1
-
-
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-
11
1
-
1
1
-
1
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1
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1
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1
8
1
1
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1
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1
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11
1
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1
1
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1
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1
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1
8
1
1
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1
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-
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702823
Kang
Production of plant-specific t ...
Capsicum annuum
Biotechnol. Lett.
31
1469-1475
2009
-
-
-
-
-
-
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1
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4
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1
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-
-
-
-
-
-
-
-
-
-
-
702831
Park
Endosperm-specific expression ...
Capsicum annuum
Biotechnol. Lett.
31
911-915
2009
-
1
1
-
1
-
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4
-
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1
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1
1
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1
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1
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-
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-
-
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-
-
-
-
-
-
-
-
673815
Niehl
-
Systemic Potato virus X infect ...
Solanum tuberosum
Funct. Plant Biol.
33
593-604
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
1
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1
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1
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1
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1
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1
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
676598
Kang
Functional analysis of the ami ...
Capsicum annuum
Plant Physiol.
140
704-715
2006
-
-
1
-
3
-
-
8
-
-
1
-
-
1
-
-
-
-
-
1
-
-
6
1
-
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-
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-
1
-
-
3
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8
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1
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-
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-
-
1
-
-
6
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
660324
Hagel
Elevated tyrosine decarboxylas ...
Nicotiana tabacum
Planta
221
904-914
2005
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
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1
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1
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1
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1
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1
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-
-
-
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-
-
-
-
-
-
660244
Jang
Production of coumaroylseroton ...
Capsicum annuum
Plant Physiol.
135
346-356
2004
-
-
-
-
-
-
1
9
-
-
-
-
-
3
-
-
1
-
-
1
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-
7
-
-
-
-
-
-
-
-
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-
-
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-
-
-
-
-
-
1
-
9
-
-
-
-
-
-
-
1
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486128
Back
Cloning and characterization o ...
Capsicum annuum
Plant Cell Physiol.
42
475-481
2001
-
-
1
-
-
-
-
-
-
-
1
1
-
5
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1
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-
5
-
-
6
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1
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1
1
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1
-
5
-
-
6
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486131
Negrel
L-Tyrosine beta-naphthylamide ...
Nicotiana tabacum, Solanum tuberosum
Phytochemistry
56
523-527
2001
-
-
-
-
-
-
8
-
-
-
-
-
-
4
-
-
2
-
-
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2
-
-
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-
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8
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8
8
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2
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-
2
-
-
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-
-
-
-
-
-
-
-
-
-
486130
Ishihara
Induction of N-hydroxycinnamoy ...
Zea mays
Biosci. Biotechnol. Biochem.
64
1025-1031
2000
-
-
-
-
-
-
2
8
-
-
1
1
-
2
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-
1
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-
1
1
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7
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2
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8
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1
1
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1
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1
1
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7
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-
-
-
-
-
-
486129
Farmer
Identification and characteriz ...
Nicotiana tabacum
Eur. J. Biochem.
263
686-694
1999
-
-
1
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3
-
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2
1
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1
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1
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2
1
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1
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2
1
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1
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2
1
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-
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-
-
-
486133
Schmidt
Cloning and expression of a po ...
Solanum tuberosum
J. Biol. Chem.
274
4273-4280
1999
-
-
1
-
-
-
-
2
-
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2
1
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4
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1
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9
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1
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2
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2
1
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1
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-
9
-
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-
-
-
-
-
-
-
-
-
-
-
-
-
486134
Yu
Purification, characterization ...
Papaver somniferum
Planta
209
33-44
1999
-
-
-
-
-
-
3
6
-
-
2
1
-
1
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1
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4
1
3
5
1
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1
1
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3
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6
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2
1
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1
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4
1
3
5
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
486132
Negrel
Purification, characterization ...
Nicotiana tabacum
Eur. J. Biochem.
247
1127-1135
1997
1
-
-
-
-
-
11
12
-
-
2
1
-
2
-
-
1
1
-
-
1
-
13
1
-
-
-
-
-
-
-
-
9
-
-
1
-
-
-
-
-
-
-
11
9
12
-
-
2
1
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-
-
1
-
-
1
-
13
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
486127
Fleurence
-
Partial purification of tyrami ...
Nicotiana tabacum
Phytochemistry
28
733-736
1989
-
-
-
-
-
2
2
4
-
-
-
1
-
1
1
-
1
-
-
1
1
2
6
-
1
-
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1
-
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-
-
-
-
-
-
-
-
-
-
2
-
2
-
4
-
-
-
1
-
1
-
1
-
1
1
2
6
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
486126
Negrel
-
The biosynthesis of feruloylty ...
Nicotiana tabacum
Phytochemistry
23
2797-2801
1984
-
-
-
-
-
1
-
6
1
1
1
1
-
1
1
-
1
-
-
1
-
1
14
-
1
-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
6
1
1
1
1
-
1
-
1
-
1
-
1
14
-
1
-
-
-
4
1
-
-
-
-
-
-
-
-