Protein Variants | Comment | Organism |
---|---|---|
D469E | formation of (3S)-1,3-dihydroxypentan-2-one in 90% enantiomeric excess in the reaction of propanal + beta-hydroxypyruvate | Escherichia coli |
D469T | formation of (3S)-1,3-dihydroxypentan-2-one in 64% enantiomeric excess in the reaction of propanal + beta-hydroxypyruvate | Escherichia coli |
D469Y | formation of (3R)-1,3-dihydroxypentan-2-one in 53% enantiomeric excess in the reaction of propanal + beta-hydroxypyruvate | Escherichia coli |
H26Y | formation of (3R)-1,3-dihydroxypentan-2-one in 88% enantiomeric excess in the reaction of propanal + beta-hydroxypyruvate | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 propanal + 2 lithium beta-hydroxypyruvate | use of lithium beta-hydroxypyruvate as a donor renders the reaction irreversible | Escherichia coli | (3S)-1,3-dihydroxypentan-2-one + (3R)-1,3-dihydroxypentan-2-one + 2 CO2 + 2 Li+ | wild-type, 58% enantiomeric excess for 3S-product | r |