Cloned (Comment) | Organism |
---|---|
1.3S subunit cloned and expressed in Escherichia coli | Propionibacterium freudenreichii subsp. shermanii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Propionibacterium freudenreichii subsp. shermanii | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | a series of truncated forms of the 1.3S subunit is prepared and the effect on the first and the second partial reaction and the overall enzyme reaction measured, result: the sequence 59-78 of 1.3S subunit is necessary for the carboxylation of biotin of the 1.3 S subunit, the inability of the truncated peptides to form enzyme complexes explains the activity loss in the overall reaction | Propionibacterium freudenreichii subsp. shermanii |
Purification (Comment) | Organism |
---|---|
recombinant enzyme and mutant 1.3S subunit | Propionibacterium freudenreichii subsp. shermanii |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate | mechanism | Propionibacterium freudenreichii subsp. shermanii | |
(S)-methylmalonyl-CoA + pyruvate = propanoyl-CoA + oxaloacetate | partial reaction 1 is catalysed specifically by the 12S subunit, partial reaction 2 is catalysed specifically by the 5S subunit | Propionibacterium freudenreichii subsp. shermanii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
propionyl-CoA + oxaloacetate | two partial reactions | Propionibacterium freudenreichii subsp. shermanii | (S)-methylmalonyl-CoA + pyruvate | - |
r |
Subunits | Comment | Organism |
---|---|---|
More | 5S subunit is a dimer of identical monomers, contains Co2+ and Zn2+ and assembles with two 1.3 subunits to form a stable complex termed the 6S subunit | Propionibacterium freudenreichii subsp. shermanii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
biotin | requirement | Propionibacterium freudenreichii subsp. shermanii |