Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
holo- and apo-isoform SHMT2, hanging drop vapor diffusion method, using 0.1 M Bis/Tris propane pH 7.5, 5% (v/v) glycerol, 12-16% (w/v) poly(ethylene glycol) 3350, 0.2 M potassium fluoride | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
tetrahydrofolate | apparent value, isoform SHMT1, at pH 7.2 and 20°C | Homo sapiens | |
0.023 | - |
tetrahydrofolate | apparent value, isoform SHMT2, at pH 7.2 and 20°C | Homo sapiens | |
0.1 | - |
L-serine | apparent value, isoform SHMT1, at pH 7.2 and 20°C | Homo sapiens | |
0.278 | - |
L-serine | apparent value, isoform SHMT2, at pH 7.2 and 20°C | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | isoform SHMT1 | Homo sapiens | 5829 | - |
mitochondrion | isoform SHMT2 | Homo sapiens | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | Homo sapiens | - |
tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | Homo sapiens | - |
5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P34896 | - |
- |
Homo sapiens | P34897 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + glycine + H2O | - |
Homo sapiens | tetrahydrofolate + L-serine | - |
r | |
tetrahydrofolate + L-serine | - |
Homo sapiens | 5,10-methylenetetrahydrofolate + glycine + H2O | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer or tetramer | isoform SHMT2, X-ray crystallography | Homo sapiens |
tetramer | isoform SHMT1, X-ray crystallography | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Homo sapiens |
SHMT1 | isoform | Homo sapiens |
SHMT2 | isoform | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.58 | - |
tetrahydrofolate | isoform SHMT1, at pH 7.2 and 20°C | Homo sapiens | |
9.58 | - |
tetrahydrofolate | isoform SHMT2, at pH 7.2 and 20°C | Homo sapiens | |
13.13 | - |
L-serine | isoform SHMT1, at pH 7.2 and 20°C | Homo sapiens | |
13.13 | - |
L-serine | isoform SHMT2, at pH 7.2 and 20°C | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | - |
Homo sapiens |