Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Geobacillus stearothermophilus |
Crystallization (Comment) | Organism |
---|---|
mutant proteins are crystallized by the hanging drop vapor diffusion method, using 50% 2-methyl 2,4-pentanediol as the precipitant with 0.2 mM EDTA, 5 mM beta-mercaptoethanol in 100 mM HEPES (pH 7.5) | Geobacillus stearothermophilus |
Protein Variants | Comment | Organism |
---|---|---|
F351G | the mutation has no effect on tetrahydrofolate-independent and tetrahydrofolate-dependent activities | Geobacillus stearothermophilus |
N341A | the mutant is inactive for the tetrahydrofolate-dependent activity, while the mutation has no effect on tetrahydrofolate-independent activity | Geobacillus stearothermophilus |
Y60A | the mutant is inactive for the tetrahydrofolate-dependent activity, while the mutation has no effect on tetrahydrofolate-independent activity | Geobacillus stearothermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.9 | - |
L-serine | wild type enzyme, at 37°C | Geobacillus stearothermophilus | |
1 | - |
L-serine | mutant enzyme F315G, at 37°C | Geobacillus stearothermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Geobacillus stearothermophilus | Q7SIB6 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, DEAE-cellulose column chromatography, gel filtration | Geobacillus stearothermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-serine + tetrahydrofolate | - |
Geobacillus stearothermophilus | glycine + 5,10-methylenetetrahydrofolate + H2O | - |
? | |
additional information | SHMT also catalyses several tetrahydrofolate-independent side reactions such as cleavage of beta-hydroxy amino acids, transamination, racemization and decarboxylation | Geobacillus stearothermophilus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | x-ray crystallography | Geobacillus stearothermophilus |
Synonyms | Comment | Organism |
---|---|---|
serine hydroxymethyltransferase | - |
Geobacillus stearothermophilus |
SHMT | - |
Geobacillus stearothermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
the melting temperature of the wild type enzyme is at 65°C | Geobacillus stearothermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
L-serine | wild type enzyme, at 37°C | Geobacillus stearothermophilus | |
3.9 | - |
L-serine | mutant enzyme F315G, at 37°C | Geobacillus stearothermophilus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyridoxal 5'-phosphate | dependent on | Geobacillus stearothermophilus |